The evolving molecular epidemiology of HIV-1 envelope subtypes in injecting drug users in Bangkok, Thailand: implications for HIV vaccine trials.

AIDS (London, England) ·Vol. 9 ·No. 8 ·1995-08-00 ·Pages 851-7

Kalish ML, Baldwin A, Raktham S, Wasi C, Luo CC, Schochetman G, Mastro TD, Young N, Vanichseni S, Rübsamen-Waigmann H

Abstract

To genetically characterize HIV-1 strains in injecting drug users (IDU) in Bangkok, Thailand in 1994, and compare these with strains found earlier in Thai IDU; such information is essential for HIV-1 vaccine development and evaluation. Peripheral blood mononuclear cells were collected from 84 IDU attending 14 drug treatment clinics in Bangkok in 1994. DNA was amplified using a nested polymerase chain reaction (PCR) procedure and sequenced directly (without cloning) from the PCR products. The V3 and flanking regions (345 nucleotides) of the env gene were analyzed using a neighbor-joining tree. Only one (1%) strain was a typical subtype B virus, 69 (82%) were genetically distinct subtype B' viruses (Thai B), and 14 (17%) were subtype E strains (Thai A). Persons with recently acquired infection were more likely to have subtype E viruses (P < 0.001) than those in our 1991 survey, who were more likely to have subtype B' viruses. Pairwise intra-subtype differences within subtypes E and B' were 5.3 and 4.3%, respectively, compared with 3.4 and 3.5% among strains collected in 1991 in Thailand. The genetic diversity within subtypes B' and E in Thailand and the proportion of new infections due to subtype E viruses among Bangkok IDU are increasing significantly. These data highlight the importance of monitoring the molecular epidemiology of HIV-1 in populations being considered for HIV-1 vaccine trials.

Keywords

Asia Behavior Developing Countries Diseases Drug Usage Examinations And Diagnoses Genetic Technics Hiv Hiv Infections Iv Drug Users Laboratory Examinations And Diagnoses Laboratory Procedures Research Report Southeastern Asia Thailand Vaccines Viral Diseases

MeSH Terms

AIDS Vaccines/pharmacology Amino Acid Sequence Base Sequence Clinical Trials as Topic DNA Primers/genetics DNA, Viral/genetics Female Genes, env Genetic Variation Glycosylation HIV Envelope Protein gp120/chemistry,genetics HIV Infections/complications,epidemiology,virology HIV-1/classification,genetics,isolation & purification Humans Male Molecular Epidemiology Molecular Sequence Data Phylogeny Sequence Homology, Amino Acid Substance Abuse, Intravenous/complications,virology Thailand/epidemiology

Chemicals

AIDS Vaccines DNA Primers DNA, Viral HIV Envelope Protein gp120

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Kalish M L

Division of HIV/AIDS, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.

Baldwin A

Raktham S

Wasi C

Luo C C

Schochetman G

Mastro T D

Young N

Vanichseni S

Rübsamen-Waigmann H

Article Info

Journal

AIDS (London, England)

Abbr.

AIDS

ISSN

0269-9370

DOI

10.1097/00002030-199508000-00004

Published

1995-08-00

Pages

851-7

Language

English

Region

England

NLM ID

8710219

Subset

Databases

GENBANK

U22542, U22543, U22544, U22545, U22546, U22547, U22548, U22549, U22550, U22551, U22552, U22553, U22554, U22555, U22556, U22557, U22558, U22559, U22560, U22561, U22562, U22563, U22564, U22565, U22566, U22567, U22568, U22569, U22570, U22571

External Links

PubMed source DOI: 10.1097/00002030-199508000-00004 View journal details

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The evolving molecular epidemiology of HIV-1 envelope subtypes in injecting drug users in Bangkok, Thailand: implications for HIV vaccine trials.

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