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PMID: 7575467 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Molecular cloning of mouse intestinal trefoil factor and its expression during goblet cell changes.

The Biochemical journal ·Vol. 311 ( Pt 1) ·1995-10-01 ·Pages 293-7

Tomita M, Itoh H, Ishikawa N, Higa A, Ide H, Murakumo Y, Maruyama H, Koga Y, Nawa Y

Abstract

A cDNA encoding mouse intestinal trefoil factor (mITF) was successfully cloned and sequenced from the small intestine of C57BL/6 mouse by using the combination of reverse transcription-PCR and rapid amplification of cDNA ends methods. The gene was, similar to rat and human ITFs, mainly expressed in the small and large intestine. The mITF expression was up-regulated during the recovery phase after depletion of goblet cells in acetic acid-induced colitis. On the other hand, the expression in the jejunum was not altered, while goblet cell hyperplasia was induced by Nippostrongylus brasiliensis infection. These results suggest that the mITF expression did not simply correlate with the number of goblet cells. The mITF may play an important role in the maintenance and repair of mucosal function of the rectum. Additionally, the mITF in the jejunum may play a role in alteration of the physicochemical nature of goblet cell mucins, thereby affecting the establishment of intestinal helminths.

MeSH Terms
Acetates Acetic Acid Amino Acid Sequence Animals Base Sequence Cloning, Molecular Colitis/chemically induced,metabolism,pathology Gene Expression Growth Substances/chemistry,genetics Humans Hyperplasia Intestine, Large/metabolism,pathology Intestine, Small/metabolism,pathology Mice Mice, Inbred C57BL Molecular Sequence Data Mucins Muscle Proteins Neuropeptides Nippostrongylus Peptides/chemistry,genetics Rats Sequence Analysis Sequence Homology Strongylida Infections/metabolism,pathology Trefoil Factor-2 Trefoil Factor-3
Chemicals
Acetates Growth Substances Mucins Muscle Proteins Neuropeptides Peptides TFF3 protein, rat Trefoil Factor-2 Trefoil Factor-3 Acetic Acid
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Tomita M
Department of Parasitology, Miyazaki Medical College, Japan.
Itoh H
Ishikawa N
Higa A
Ide H
Murakumo Y
Maruyama H
Koga Y
Nawa Y
References (29)
29 references, click to expand
  1. Goblet cell mucins as the selective barrier for the intestinal helminths: T-cell-independent alteration of goblet cell mucins by immunologically 'damaged' Nippostrongylus brasiliensis worms and its significance on the challenge infection with homologous and heterologous parasites.
    Immunology. 1994 Mar;81(3):480-6 PMID: 8206520
  2. Trefoil peptides promote epithelial migration through a transforming growth factor beta-independent pathway.
    J Clin Invest. 1994 Jul;94(1):376-83 PMID: 8040278
  3. Reconstitution by bone marrow grafting of the defective protective capacity at the migratory phase but not at the intestinal phase of Nippostrongylus brasiliensis infection in W/Wv mice.
    Parasite Immunol. 1994 Apr;16(4):181-6 PMID: 8058356
  4. Inhibitory effects of concurrently present 'normal' Nippostrongylus brasiliensis worms on expulsion of 'damaged' worms and associated goblet cell changes in rats.
    Parasite Immunol. 1994 Jun;16(6):329-32 PMID: 7970871
  5. Selective effector mechanisms for the expulsion of intestinal helminths.
    Parasite Immunol. 1994 Jul;16(7):333-8 PMID: 7970872
  6. Nippostrongylus brasiliensis: intestinal goblet-cell response in adoptively immunized rats.
    Exp Parasitol. 1979 Feb;47(1):81-90 PMID: 421768
  7. How signal sequences maintain cleavage specificity.
    J Mol Biol. 1984 Feb 25;173(2):243-51 PMID: 6423828
  8. hSP, the domain-duplicated homolog of pS2 protein, is co-expressed with pS2 in stomach but not in breast carcinoma.
    EMBO J. 1990 Feb;9(2):407-14 PMID: 2303034
  9. Short chain fatty acid-induced colitis in mice.
    Int J Tissue React. 1989;11(4):165-8 PMID: 2634625
  10. Molecular cloning of rat intestinal mucin. Lack of conservation between mammalian species.
    J Biol Chem. 1991 Nov 25;266(33):22733-8 PMID: 1939280
  11. Identification and characterization of rat intestinal trefoil factor: tissue- and cell-specific member of the trefoil protein family.
    Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11017-21 PMID: 1763017
  12. Localization of intestinal trefoil-factor mRNA in rat stomach and intestine by hybridization in situ.
    Biochem J. 1992 Jul 1;285 ( Pt 1):5-8 PMID: 1637322
  13. Mucosal protective activity of prostaglandin analogs in rodent colonic inflammation.
    Inflammation. 1992 Dec;16(6):623-9 PMID: 1334049
  14. Trefoil peptide gene expression in gastrointestinal epithelial cells in inflammatory bowel disease.
    Gastroenterology. 1993 Jan;104(1):12-20 PMID: 8419234
  15. Identification of human intestinal trefoil factor. Goblet cell-specific expression of a peptide targeted for apical secretion.
    J Biol Chem. 1993 Mar 25;268(9):6694-702 PMID: 8454642
  16. Immune-mediated alteration of the terminal sugars of goblet cell mucins in the small intestine of Nippostrongylus brasiliensis-infected rats.
    Immunology. 1993 Feb;78(2):303-7 PMID: 8473019
  17. Trefoil peptides and the gut.
    Gut. 1993 May;34(5):577-9 PMID: 8504953
  18. Rapid amplification of complementary DNA ends for generation of full-length complementary DNAs: thermal RACE.
    Methods Enzymol. 1993;218:340-56 PMID: 7685466
  19. The mouse one P-domain (pS2) and two P-domain (mSP) genes exhibit distinct patterns of expression.
    J Cell Biol. 1993 Jul;122(1):191-8 PMID: 8314841
  20. hP1.B, a human P-domain peptide homologous with rat intestinal trefoil factor, is expressed also in the ulcer-associated cell lineage and the uterus.
    Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):6961-5 PMID: 8346203
  21. Trefoil peptides: a newly recognized family of epithelial mucin-associated molecules.
    Am J Physiol. 1993 Aug;265(2 Pt 1):G205-13 PMID: 8368306
  22. The P-domain or trefoil motif: a role in renewal and pathology of mucous epithelia?
    Trends Biochem Sci. 1993 Jul;18(7):239-43 PMID: 8267796
  23. Trefoil peptide gene expression in small intestinal Crohn's disease and dietary adaptation.
    J Clin Gastroenterol. 1993;17 Suppl 1:S78-91 PMID: 8283019
  24. A glucocorticoid prodrug facilitates normal mucosal function in rat colitis without adrenal suppression.
    Gastroenterology. 1994 Feb;106(2):405-13 PMID: 7507873
  25. Mast cell protease inhibitor, trypstatin, is a fragment of inter-alpha-trypsin inhibitor light chain.
    J Biol Chem. 1994 Feb 4;269(5):3818-22 PMID: 7508921
  26. Preferential alternative splicing in cancer generates a K-sam messenger RNA with higher transforming activity.
    Cancer Res. 1994 Jun 15;54(12):3237-41 PMID: 8205545
  27. Sequencing of cDNAs encoding alpha 1-microglobulin/bikunin of Mongolian gerbil and Syrian golden hamster in comparison with man and other species.
    Biochim Biophys Acta. 1994 Dec 14;1209(2):286-92 PMID: 7529051
  28. cDNA sequencing of mouse alpha 1-microglobulin/inter-alpha-trypsin inhibitor light chain and its expression in acute inflammation.
    J Biochem. 1994 Oct;116(4):767-72 PMID: 7533761
  29. Histochemical characteristics of the goblet cell mucins and their role in defence mechanisms against Nippostrongylus brasiliensis infection in the small intestine of mice.
    Parasite Immunol. 1994 Dec;16(12):649-54 PMID: 7708431
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj3110293
Published
1995-10-01
Pages
293-7
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1136151
Subset
IM
Databases
GENBANK
D38410
External Links
PubMed source DOI: 10.1042/bj3110293 PMC Full Text View journal details
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