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PMID: 75712 Published · ppublish English Journal Article

Actinomycin biosynthesis by protoplasts derived from Streptomyces parvulus.

Antimicrobial agents and chemotherapy ·Vol. 13 ·No. 1 ·1978-01-00 ·Pages 104-14

Hitchcock MJ, Katz E

Abstract

Conditions are described for the formation of protoplasts from Streptomyces parvulus that are able to synthesize actinomycin D de novo. Antibiotic synthesis by protoplasts, in contrast to that by mycelium, was sensitive to inhibition by actinomycin D and to a decrease in sucrose concentration. On the other hand, synthesis by mycelium was much more sensitive to inhibition by amino acid analogs (d-valine, cis-3-methylproline, and alpha-methyl-dl-tryptophan). In addition, the uptake of amino acids (l-methionine, sarcosine, and l- and d-valine) by protoplasts was significantly lower than that by mycelium. The advantages and limitations of using protoplasts for studying in vivo actinomycin synthesis are discussed.

MeSH Terms
Dactinomycin/biosynthesis Protoplasts/metabolism Streptomyces/metabolism
Chemicals
Dactinomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hitchcock M J
Katz E
References (37)
37 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.13.1.104
Published
1978-01-00
Pages
104-14
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC352192
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.13.1.104 PMC Full Text View journal details
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