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PMID: 7568200 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Spatial learning with a minislab in the dorsal hippocampus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 21 ·1995-10-10 ·Pages 9697-701

Moser MB, Moser EI, Forrest E, Andersen P, Morris RG

Abstract

We have determined the volume and location of hippocampal tissue required for normal acquisition of a spatial memory task. Ibotenic acid was used to make bilateral symmetric lesions of 20-100% of hippocampal volume. Even a small transverse block (minislab) of the hippocampus (down to 26% of the total) could support spatial learning in a water maze, provided it was at the septal (dorsal) pole of the hippocampus. Lesions of the septal pole, leaving 60% of the hippocampi intact, caused a learning deficit, although normal electrophysiological responses, synaptic plasticity, and preserved acetylcholinesterase staining argue for adequate function of the remaining tissue. Thus, with an otherwise normal brain, hippocampal-dependent spatial learning only requires a minislab of dorsal hippocampal tissue.

MeSH Terms
Acetylcholinesterase/isolation & purification Animals Electric Stimulation Electrophysiology Excitatory Amino Acid Agonists/pharmacology Hippocampus/drug effects,pathology,physiology Histocytochemistry Ibotenic Acid/pharmacology Learning Maze Learning/drug effects,physiology Rats Rats, Inbred Strains Spatial Behavior/drug effects,physiology Swimming
Chemicals
Excitatory Amino Acid Agonists Ibotenic Acid Acetylcholinesterase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Moser M B
Centre for Neuroscience, University of Edinburgh, United Kingdom.
Moser E I
Forrest E
Andersen P
Morris R G
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.21.9697
Published
1995-10-10
Pages
9697-701
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC40869
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.92.21.9697 PMC Full Text View journal details
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