Home LiteratureArticle Details
PMID: 7568077 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Telomerase activity in normal and malignant hematopoietic cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 20 ·1995-09-26 ·Pages 9082-6

Broccoli D, Young JW, de Lange T

Abstract

Bone marrow and peripheral blood leukocytes from 19 leukemia patients were found to contain telomerase activity detectable by a PCR-based assay. Telomerase was also detectable in nonmalignant bone marrow and peripheral blood leukocytes from normal donors, including fractions enriched for granulocytes, T lymphocytes, and monocytes/B cells. Semiquantitative comparison revealed considerable overlap between telomerase activities in samples from normal subjects and leukemia patients, confounding evaluation of the role of telomerase in this disease. These data indicate that human telomerase is not restricted to immortal cells and suggest that the somatic expression of this enzyme may be more widespread than was previously inferred from the decline of human telomeres.

MeSH Terms
Acute Disease B-Lymphocytes/enzymology Base Sequence Bone Marrow/enzymology,pathology DNA Primers Granulocytes/enzymology Hematopoietic Stem Cells/enzymology Humans Kinetics Leukemia/blood,enzymology,pathology Leukemia, Myelogenous, Chronic, BCR-ABL Positive/enzymology Lymphocytes/enzymology Molecular Sequence Data Polymerase Chain Reaction/methods Reference Values Remission Induction T-Lymphocytes/enzymology Telomerase/analysis,blood,metabolism
Chemicals
DNA Primers Telomerase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Broccoli D
Laboratory for Cell Biology and Genetics, Rockefeller University, New York, NY 10021, USA.
Young J W
de Lange T
References (22)
22 references, click to expand
  1. Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n.
    Mol Cell Biol. 1993 Jul;13(7):4301-10 PMID: 8321232
  2. E6 of human papillomavirus type 16 can overcome the M1 stage of immortalization in human mammary epithelial cells but not in human fibroblasts.
    Oncogene. 1993 Jun;8(6):1407-13 PMID: 8389027
  3. Stabilization of short telomeres and telomerase activity accompany immortalization of Epstein-Barr virus-transformed human B lymphocytes.
    J Virol. 1994 May;68(5):3410-4 PMID: 8151802
  4. Activation of telomerase in a human tumor.
    Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):2882-5 PMID: 8159672
  5. Telomerase activity in human ovarian carcinoma.
    Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):2900-4 PMID: 8159676
  6. Mammalian telomere dynamics: healing, fragmentation shortening and stabilization.
    Curr Opin Genet Dev. 1994 Apr;4(2):203-11 PMID: 8032197
  7. Evidence for a mitotic clock in human hematopoietic stem cells: loss of telomeric DNA with age.
    Proc Natl Acad Sci U S A. 1994 Oct 11;91(21):9857-60 PMID: 7937905
  8. Specific association of human telomerase activity with immortal cells and cancer.
    Science. 1994 Dec 23;266(5193):2011-5 PMID: 7605428
  9. Telomerase activity in normal leukocytes and in hematologic malignancies.
    Blood. 1995 May 1;85(9):2315-20 PMID: 7727765
  10. Sugar and disease.
    Nature. 1972 Sep 22;239(5369):197-9 PMID: 4263504
  11. Variability at the telomeres of the human X/Y pseudoautosomal region.
    Cold Spring Harb Symp Quant Biol. 1986;51 Pt 1:213-9 PMID: 3034483
  12. Telomeric repeat from T. thermophila cross hybridizes with human telomeres.
    Nature. 1988 Apr 14;332(6165):656-9 PMID: 2833706
  13. The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats.
    Cell. 1989 Nov 3;59(3):521-9 PMID: 2805070
  14. Structure and variability of human chromosome ends.
    Mol Cell Biol. 1990 Feb;10(2):518-27 PMID: 2300052
  15. Telomeres shorten during ageing of human fibroblasts.
    Nature. 1990 May 31;345(6274):458-60 PMID: 2342578
  16. Telomere reduction in human colorectal carcinoma and with ageing.
    Nature. 1990 Aug 30;346(6287):866-8 PMID: 2392154
  17. Recognition of a chromosome truncation site associated with alpha-thalassaemia by human telomerase.
    Nature. 1991 Oct 3;353(6343):454-6 PMID: 1896089
  18. Telomere loss: mitotic clock or genetic time bomb?
    Mutat Res. 1991 Mar-Nov;256(2-6):271-82 PMID: 1722017
  19. Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity.
    EMBO J. 1992 May;11(5):1921-9 PMID: 1582420
  20. Telomere length predicts replicative capacity of human fibroblasts.
    Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10114-8 PMID: 1438199
  21. Loss of telomeric DNA during aging of normal and trisomy 21 human lymphocytes.
    Am J Hum Genet. 1993 Apr;52(4):661-7 PMID: 8460632
  22. Restoration of telomeres in human papillomavirus-immortalized human anogenital epithelial cells.
    Mol Cell Biol. 1994 Feb;14(2):961-9 PMID: 8289836
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.20.9082
Published
1995-09-26
Pages
9082-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC40928
Subset
IM
Grants
NIAID NIH HHS · AI26875 · United States
NCI NIH HHS · CA23766 · United States
NIGMS NIH HHS · GM49046 · United States
External Links
PubMed source DOI: 10.1073/pnas.92.20.9082 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2