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PMID: 7567990 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Retinoid X receptor alpha forms tetramers in solution.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 19 ·1995-09-12 ·Pages 8645-9

Kersten S, Kelleher D, Chambon P, Gronemeyer H, Noy N

Abstract

Protein-protein interactions allow the retinoid X receptor (RXR) to bind to cognate DNA as a homo- or a heterodimer and to participate in mediating the effects of a variety of hormones on gene transcription. Here we report a systematic study of the oligomeric state of RXR in the absence of a DNA template. We have used electrophoresis under nondenaturing conditions and chemical crosslinking to show that in solution, RXR alpha forms homodimers as well as homotetramers. The dissociation constants governing dimer and tetramer formation were estimated by fluorescence anisotropy studies. The results indicate that RXR tetramers are formed with a high affinity and that at protein concentrations higher than about 70 nM, tetramers will constitute the predominant species. Tetramer formation may provide an additional level of the regulation of gene transcription mediated by RXRs.

MeSH Terms
Base Sequence Cross-Linking Reagents Electrophoresis, Polyacrylamide Gel Escherichia coli/genetics Fluorescence Polarization Fluorescent Dyes Molecular Sequence Data Peptide Fragments/chemistry Protein Conformation Protein Denaturation Receptors, Retinoic Acid/chemistry Recombinant Proteins/chemistry Retinoid X Receptors Sequence Deletion Solutions Transcription Factors/chemistry
Chemicals
Cross-Linking Reagents Fluorescent Dyes Peptide Fragments Receptors, Retinoic Acid Recombinant Proteins Retinoid X Receptors Solutions Transcription Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kersten S
Cornell University, Division of Nutritional Sciences, Ithaca, NY 14853-6301, USA.
Kelleher D
Chambon P
Gronemeyer H
Noy N
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.19.8645
Published
1995-09-12
Pages
8645-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC41023
Subset
IM
Grants
NIDDK NIH HHS · DK42601 · United States
External Links
PubMed source DOI: 10.1073/pnas.92.19.8645 PMC Full Text View journal details
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