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PMID: 7558319 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Escherichia coli induces transuroepithelial neutrophil migration by an intercellular adhesion molecule-1-dependent mechanism.

Infection and immunity ·Vol. 63 ·No. 10 ·1995-10-00 ·Pages 4054-62

Agace WW, Patarroyo M, Svensson M, Carlemalm E, Svanborg C

Abstract

During bacterial infections at mucosal sites, neutrophils migrate to the mucosa and cross the epithelial barrier. We have examined neutrophil migration across Escherichia coli-stimulated uroepithelial cell layers in an attempt to more fully understand this process. Stimulation of uroepithelial cells with E. coli or interleukin-1 alpha (IL-1 alpha) induced transepithelial neutrophil migration in a time- and stimulant dose-dependent manner. Uroepithelial cell lines and nontransformed uroepithelial cells expressed intercellular adhesion molecule-1 (ICAM-1) but not ICAM-2, E-selectin, or P-selectin. Epithelial ICAM-1 expression was enhanced after stimulation with E. coli or IL-1 alpha. Anti-ICAM-1 antibody reduced transepithelial neutrophil migration by 61 to 85%, indicating that neutrophils bound ICAM-1 on the epithelial surface. Antibodies to CD18 and CD11b reduced migration by 70 to 79%, suggesting that CD11b/CD18 (Mac-1) was acting as the neutrophil receptor for ICAM-1 in this process. Anti-CD11a antibodies had no effect on neutrophil migration. In conclusion, E. coli induced ICAM-1- and Mac-1-dependent transepithelial neutrophil migration. Previous studies have shown that urinary tract epithelial cells secrete IL-8 when exposed to E. coli or IL-1 alpha. These observations suggest that epithelial cells play an active role in neutrophil migration during urinary tract infections.

MeSH Terms
CD18 Antigens/physiology Cell Movement Escherichia coli/physiology Female Humans Intercellular Adhesion Molecule-1/physiology Lipopolysaccharides/toxicity Neutrophils/physiology Urinary Tract/immunology Urinary Tract Infections/immunology
Chemicals
CD18 Antigens Lipopolysaccharides Intercellular Adhesion Molecule-1
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Agace W W
Department of Medical Microbiology, Lund University, Sweden.
Patarroyo M
Svensson M
Carlemalm E
Svanborg C
References (35)
35 references, click to expand
  1. Neutrophil migration across a cultured intestinal epithelium. Dependence on a CD11b/CD18-mediated event and enhanced efficiency in physiological direction.
    J Clin Invest. 1991 Nov;88(5):1605-12 PMID: 1682344
  2. The expression of human intercellular adhesion molecule-2 is refractory to inflammatory cytokines.
    Eur J Immunol. 1991 Oct;21(10):2629-32 PMID: 1680706
  3. Involvement of beta 2-integrins in the migration of human natural killer cells.
    J Immunol. 1992 Jul 15;149(2):590-8 PMID: 1378070
  4. Selective cytokine production by epithelial cells following exposure to Escherichia coli.
    Infect Immun. 1993 Feb;61(2):602-9 PMID: 8423089
  5. Neutrophil migration across cultured intestinal epithelial monolayers is modulated by epithelial exposure to IFN-gamma in a highly polarized fashion.
    J Cell Biol. 1993 Feb;120(3):785-98 PMID: 8093887
  6. Selectins.
    J Clin Invest. 1993 Feb;91(2):379-87 PMID: 7679406
  7. Cyclosporin A does not inhibit IL-1 alpha-induced epithelial cell IL-6 secretion.
    Scand J Immunol. 1993 May;37(5):581-6 PMID: 8484104
  8. Effects of Escherichia coli and E. coli lipopolysaccharides on the function of human ureteral epithelial cells cultured in serum-free medium.
    Infect Immun. 1993 Aug;61(8):3304-12 PMID: 8101508
  9. Interleukin-8 and the neutrophil response to mucosal gram-negative infection.
    J Clin Invest. 1993 Aug;92(2):780-5 PMID: 8349817
  10. Salmonella typhimurium attachment to human intestinal epithelial monolayers: transcellular signalling to subepithelial neutrophils.
    J Cell Biol. 1993 Nov;123(4):895-907 PMID: 8227148
  11. Neutrophils roll on E-selectin.
    J Immunol. 1993 Dec 1;151(11):6338-46 PMID: 7504018
  12. Uroepithelial cells are part of a mucosal cytokine network.
    Infect Immun. 1994 Jun;62(6):2315-21 PMID: 8188354
  13. Epithelial glucosphingolipid expression as a determinant of bacterial adherence and cytokine production.
    Infect Immun. 1994 Oct;62(10):4404-10 PMID: 7927702
  14. Constitutive alpha V beta 3 integrin-mediated adhesion of human lymphoid B cells to vitronectin substrate.
    Cell Immunol. 1995 Feb;160(2):165-72 PMID: 7536632
  15. Colo-rectal biopsy in inflammatory bowel disease.
    Monogr Pathol. 1977;(18):50-94 PMID: 611415
  16. Inhibition of experimental ascending urinary tract infection by an epithelial cell-surface receptor analogue.
    Nature. 1982 Aug 5;298(5874):560-2 PMID: 7048106
  17. Lung neutrophils in the adult respiratory distress syndrome. Clinical and pathophysiologic significance.
    Am Rev Respir Dis. 1986 Feb;133(2):218-25 PMID: 3004270
  18. Two distinct monokines, interleukin 1 and tumor necrosis factor, each independently induce biosynthesis and transient expression of the same antigen on the surface of cultured human vascular endothelial cells.
    J Immunol. 1986 Mar 1;136(5):1680-7 PMID: 3485132
  19. Overlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor, and immune interferon.
    J Immunol. 1986 Sep 15;137(6):1893-6 PMID: 3091693
  20. Identification of a novel adhesion molecule in human leukocytes by monoclonal antibody LB-2.
    FEBS Lett. 1987 Jan 5;210(2):127-31 PMID: 3792557
  21. Neutrophil recruitment and bacterial clearance correlated with LPS responsiveness in local gram-negative infection.
    J Immunol. 1987 May 15;138(10):3475-80 PMID: 3553327
  22. Cellular basis of host defence in pyelonephritis. III. Deletion of individual components.
    Br J Exp Pathol. 1987 Jun;68(3):377-88 PMID: 3040066
  23. Effects of polymorphonuclear leukocyte transmigration on the barrier function of cultured intestinal epithelial monolayers.
    J Clin Invest. 1987 Oct;80(4):1104-13 PMID: 3116044
  24. Purified intercellular adhesion molecule-1 (ICAM-1) is a ligand for lymphocyte function-associated antigen 1 (LFA-1).
    Cell. 1987 Dec 4;51(5):813-9 PMID: 3315233
  25. Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins.
    Science. 1989 Mar 3;243(4895):1160-5 PMID: 2466335
  26. MALA-2, mouse homologue of human adhesion molecule ICAM-1 (CD54).
    Eur J Immunol. 1989 Sep;19(9):1551-7 PMID: 2571505
  27. Effects of anti-inflammatory agents on mucosal inflammation induced by infection with gram-negative bacteria.
    Infect Immun. 1990 Jul;58(7):2056-60 PMID: 2194956
  28. Interleukin-6 response to deliberate colonization of the human urinary tract with gram-negative bacteria.
    Infect Immun. 1991 Jan;59(1):421-7 PMID: 1987054
  29. ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18).
    J Cell Biol. 1990 Dec;111(6 Pt 2):3129-39 PMID: 1980124
  30. Role of the adhesion molecule ICAM-1 (CD54) in staphylococcal enterotoxin-mediated cytotoxicity.
    Eur J Immunol. 1991 Jan;21(1):131-5 PMID: 1671356
  31. Cytokine-activated human endothelial monolayers support enhanced neutrophil transmigration via a mechanism involving both endothelial-leukocyte adhesion molecule-1 and intercellular adhesion molecule-1.
    J Immunol. 1991 Mar 1;146(5):1617-25 PMID: 1704400
  32. Cytokine regulation of ICAM-1 expression on human renal tubular epithelial cells in vitro.
    Transplantation. 1991 Jun;51(6):1272-5 PMID: 1675504
  33. Characterization of ICAM-2 and evidence for a third counter-receptor for LFA-1.
    J Exp Med. 1991 Jul 1;174(1):253-67 PMID: 1676048
  34. Persistence of Escherichia coli bacteriuria is not determined by bacterial adherence.
    Infect Immun. 1991 Sep;59(9):2915-21 PMID: 1879917
  35. Interleukin-6 response of epithelial cell lines to bacterial stimulation in vitro.
    Infect Immun. 1992 Apr;60(4):1295-301 PMID: 1347759
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.63.10.4054-4062.1995
Published
1995-10-00
Pages
4054-62
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC173570
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.63.10.4054-4062.1995 PMC Full Text View journal details
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