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PMID: 7545288 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A simple procedure for gel electrophoresis and northern blotting of RNA.

Nucleic acids research ·Vol. 23 ·No. 16 ·1995-08-25 ·Pages 3357-8

Goda SK, Minton NP

Abstract

暂无摘要

MeSH Terms
Blotting, Northern/methods Clostridium/chemistry Electrophoresis, Agar Gel/methods Guanidines RNA/isolation & purification RNA, Bacterial/isolation & purification Thiocyanates
Chemicals
Guanidines RNA, Bacterial Thiocyanates guanidine thiocyanate RNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Goda S K
Department of Molecular Microbiology, Centre for Applied Microbiology and Research, Salisbury, Wiltshire, UK.
Minton N P
References (7)
7 references, click to expand
  1. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 PMID: 73185
  2. Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels.
    Nucleic Acids Res. 1979 Aug 10;6(11):3559-67 PMID: 573889
  3. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  4. The complete amino acid sequence of the Clostridium botulinum type A neurotoxin, deduced by nucleotide sequence analysis of the encoding gene.
    Eur J Biochem. 1990 Apr 20;189(1):73-81 PMID: 2185020
  5. Specificity of oligo (dT)-cellulose chromatography in the isolation of polyadenylated RNA.
    Anal Biochem. 1976 May 7;72:413-27 PMID: 782287
  6. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
    Anal Biochem. 1976 Jan;70(1):75-85 PMID: 1259158
  7. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
    Biochemistry. 1977 Oct 18;16(21):4743-51 PMID: 911786
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/23.16.3357
Published
1995-08-25
Pages
3357-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC307204
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/23.16.3357 PMC Full Text View journal details
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