Home LiteratureArticle Details
PMID: 7544118 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Effect of transforming growth factor-beta 1 and basic fibroblast growth factor on the expression of cell surface proteoglycans in human lung fibroblasts. Enhanced glycanation and fibronectin-binding of CD44 proteoglycan, and down-regulation of glypican.

The Biochemical journal ·Vol. 310 ( Pt 1) ·1995-08-15 ·Pages 73-81

Romarís M, Bassols A, David G

Abstract

We have tested the effects of transforming growth factor-beta 1 (TGF-beta 1), basic fibroblast growth factor (bFGF) and TGF-beta 1 + bFGF on the expression of the cell surface proteoglycans (CD44, syndecans and glypican) in cultures of human lung fibroblasts (HLF). Cell surface proteoglycan expression was monitored by quantitative immunoprecipitation from metabolically labelled cells. Western and Northern blotting and evaluation of the glycanation of the proteoglycans. Stimulation of the cells with TGF-beta 1 increased the length of the chondroitin sulphate (CS) chains on CD44 (approximately 1.6-fold). bFGF, administered solely, also increased the length of the CS chains on CD44 (approximately 1.4-fold), whereas the combination of TGF-beta 1 + bFGF nearly doubled both the length and the number of the CS chains on CD44. None of these treatments lead to changes in CD44 message or core-protein expression. This enhanced glycanation of CD44 after the TGF-beta 1, bFGF and combined treatments correlated with a 2-fold increase in the affinity of the proteoglycan for fibronectin but had no influence on the binding to type I collagen. TGF-beta 1, alone or in combination with bFGF, also stimulated the CS content of syndecan-1, but none of the other syndecans was significantly affected by any of the factors or combinations tested. The expression of glypican however was significantly decreased (nearly halved) by the combination of TGF-beta 1 + bFGF, less so by TGF-beta 1 and not at all by bFGF. This decrease occurred both at the level of the message and of the core protein. These data demonstrate specific and differential effects of TGF-beta 1 and bFGF on the structure, expression and interactions of the cell surface proteoglycans of HLF.

MeSH Terms
Base Sequence Carrier Proteins/genetics,metabolism Cell Membrane/metabolism Cells, Cultured DNA, Complementary Down-Regulation Fibroblast Growth Factor 2/pharmacology Fibronectins/metabolism Heparan Sulfate Proteoglycans Heparitin Sulfate/biosynthesis,metabolism Humans Hyaluronan Receptors Lung/drug effects,metabolism Molecular Sequence Data Polysaccharides/metabolism Protein Binding Proteoglycans/biosynthesis,metabolism Receptors, Cell Surface/genetics,metabolism Receptors, Lymphocyte Homing/genetics,metabolism Transforming Growth Factor beta/pharmacology
Chemicals
Carrier Proteins DNA, Complementary Fibronectins Heparan Sulfate Proteoglycans Hyaluronan Receptors Polysaccharides Proteoglycans Receptors, Cell Surface Receptors, Lymphocyte Homing Transforming Growth Factor beta Fibroblast Growth Factor 2 Heparitin Sulfate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Romarís M
Center for Human Genetics, University of Leuven, Belgium.
Bassols A
David G
References (57)
57 references, click to expand
  1. Transforming growth factor beta regulates the expression and structure of extracellular matrix chondroitin/dermatan sulfate proteoglycans.
    J Biol Chem. 1988 Feb 25;263(6):3039-45 PMID: 3422640
  2. Proteoglycan forms of the lymphocyte homing receptor CD44 are alternatively spliced variants containing the v3 exon.
    J Cell Biol. 1995 Feb;128(4):673-85 PMID: 7532175
  3. Transforming growth factor beta stimulates the expression of fibronectin and of both subunits of the human fibronectin receptor by cultured human lung fibroblasts.
    J Biol Chem. 1988 Apr 5;263(10):4586-92 PMID: 2965146
  4. The high molecular weight receptor to transforming growth factor-beta contains glycosaminoglycan chains.
    J Biol Chem. 1988 Jun 15;263(17):8366-70 PMID: 2897367
  5. Biochemical properties of glycoproteins involved in lymphocyte recognition of high endothelial venules in man.
    J Immunol. 1988 Sep 1;141(5):1615-23 PMID: 3137259
  6. Altered structure of the hybrid cell surface proteoglycan of mammary epithelial cells in response to transforming growth factor-beta.
    J Cell Biol. 1988 Nov;107(5):1959-67 PMID: 3141430
  7. Regulation of cell adhesion receptors by transforming growth factor-beta. Concomitant regulation of integrins that share a common beta 1 subunit.
    J Biol Chem. 1989 Jan 5;264(1):380-8 PMID: 2491849
  8. Membrane-associated chondroitin sulfate proteoglycans of human lung fibroblasts.
    J Cell Biol. 1989 Mar;108(3):1165-73 PMID: 2646307
  9. A lymphocyte molecule implicated in lymph node homing is a member of the cartilage link protein family.
    Cell. 1989 Mar 24;56(6):1057-62 PMID: 2466575
  10. A human lymphocyte homing receptor, the hermes antigen, is related to cartilage proteoglycan core and link proteins.
    Cell. 1989 Mar 24;56(6):1063-72 PMID: 2466576
  11. Multiple distinct membrane heparan sulfate proteoglycans in human lung fibroblasts.
    J Biol Chem. 1989 Apr 25;264(12):7009-16 PMID: 2523387
  12. Partial primary structure of the 48- and 90-kilodalton core proteins of cell surface-associated heparan sulfate proteoglycans of lung fibroblasts. Prediction of an integral membrane domain and evidence for multiple distinct core proteins at the cell surface of human lung fibroblasts.
    J Biol Chem. 1989 Apr 25;264(12):7017-24 PMID: 2523388
  13. Transforming growth factor (type beta) promotes the addition of chondroitin sulfate chains to the cell surface proteoglycan (syndecan) of mouse mammary epithelia.
    J Cell Biol. 1989 Nov;109(5):2509-18 PMID: 2509487
  14. Multiple domains of the large fibroblast proteoglycan, versican.
    EMBO J. 1989 Oct;8(10):2975-81 PMID: 2583089
  15. Analysis of glycosaminoglycan substitution in decorin by site-directed mutagenesis.
    J Biol Chem. 1990 Mar 25;265(9):5317-23 PMID: 2108171
  16. Characteristics of the in vitro interaction of a small proteoglycan (PG II) of bovine tendon with type I collagen.
    Matrix. 1989;9(6):468-78 PMID: 2635760
  17. The extended family of proteoglycans: social residents of the pericellular zone.
    Curr Opin Cell Biol. 1989 Dec;1(6):1201-18 PMID: 2517581
  18. CD44 is the principal cell surface receptor for hyaluronate.
    Cell. 1990 Jun 29;61(7):1303-13 PMID: 1694723
  19. Negative regulation of transforming growth factor-beta by the proteoglycan decorin.
    Nature. 1990 Jul 19;346(6281):281-4 PMID: 2374594
  20. Transforming growth factor beta alters the expression of heparan sulfate proteoglycan in human colon carcinoma cells.
    J Biol Chem. 1990 Oct 15;265(29):18023-9 PMID: 1698783
  21. Hyaluronic acid synthesis by mural granulosa cells and cumulus cells in vitro is selectively stimulated by a factor produced by oocytes and by transforming growth factor-beta.
    J Biol Chem. 1990 Nov 15;265(32):19517-23 PMID: 2123188
  22. Molecular cloning of a phosphatidylinositol-anchored membrane heparan sulfate proteoglycan from human lung fibroblasts.
    J Cell Biol. 1990 Dec;111(6 Pt 2):3165-76 PMID: 2148568
  23. The transforming growth factor-beta family.
    Annu Rev Cell Biol. 1990;6:597-641 PMID: 2177343
  24. Human keratinocytes express a new CD44 core protein (CD44E) as a heparan-sulfate intrinsic membrane proteoglycan with additional exons.
    J Cell Biol. 1991 Apr;113(1):207-21 PMID: 2007624
  25. Differential regulation of extracellular matrix proteoglycan (PG) gene expression. Transforming growth factor-beta 1 up-regulates biglycan (PGI), and versican (large fibroblast PG) but down-regulates decorin (PGII) mRNA levels in human fibroblasts in culture.
    J Biol Chem. 1991 Jun 5;266(16):10608-15 PMID: 2037600
  26. Cytokines in context.
    J Cell Biol. 1991 Jun;113(5):981-6 PMID: 2040651
  27. Differential effect of transforming growth factor beta on proteoglycan synthesis in human embryonic lung fibroblasts.
    Biochim Biophys Acta. 1991 Jul 10;1093(2-3):229-33 PMID: 1863601
  28. Proteoglycans: structures and interactions.
    Annu Rev Biochem. 1991;60:443-75 PMID: 1883201
  29. Effects of platelet-derived growth factor and transforming growth factor-beta 1 on the synthesis of a large versican-like chondroitin sulfate proteoglycan by arterial smooth muscle cells.
    J Biol Chem. 1991 Sep 15;266(26):17640-7 PMID: 1894644
  30. Genetic analysis of proteoglycan structure, function and metabolism.
    Curr Opin Cell Biol. 1991 Oct;3(5):805-16 PMID: 1931081
  31. Structure and expression of the membrane proteoglycan betaglycan, a component of the TGF-beta receptor system.
    Cell. 1991 Nov 15;67(4):785-95 PMID: 1657406
  32. Expression cloning and characterization of the TGF-beta type III receptor.
    Cell. 1991 Nov 15;67(4):797-805 PMID: 1657407
  33. Differential expression of cell surface heparan sulfate proteoglycans in human mammary epithelial cells and lung fibroblasts.
    J Biol Chem. 1992 Jan 15;267(2):1116-22 PMID: 1339431
  34. A cell surface chondroitin sulfate proteoglycan, immunologically related to CD44, is involved in type I collagen-mediated melanoma cell motility and invasion.
    J Cell Biol. 1992 Jan;116(2):521-31 PMID: 1730766
  35. Lymphocyte CD44 binds the COOH-terminal heparin-binding domain of fibronectin.
    J Cell Biol. 1992 Feb;116(3):817-25 PMID: 1730778
  36. Growth factors induce 3T3 cells to express bFGF-binding syndecan.
    J Biol Chem. 1992 Mar 25;267(9):6435-41 PMID: 1556147
  37. Cell surface heparan sulfate proteoglycans.
    J Biol Chem. 1992 May 15;267(14):9451-4 PMID: 1577788
  38. Cell surface phosphatidylinositol-anchored heparan sulfate proteoglycan initiates mouse melanoma cell adhesion to a fibronectin-derived, heparin-binding synthetic peptide.
    J Cell Biol. 1992 Jun;117(6):1331-41 PMID: 1607392
  39. Transforming growth factor-beta in disease: the dark side of tissue repair.
    J Clin Invest. 1992 Jul;90(1):1-7 PMID: 1634602
  40. Molecular cloning of amphiglycan, a novel integral membrane heparan sulfate proteoglycan expressed by epithelial and fibroblastic cells.
    J Cell Biol. 1992 Aug;118(4):961-9 PMID: 1500433
  41. Biology of the syndecans: a family of transmembrane heparan sulfate proteoglycans.
    Annu Rev Cell Biol. 1992;8:365-93 PMID: 1335744
  42. Formation of anhydrosugars in the chemical depolymerization of heparin.
    Biochemistry. 1976 Sep 7;15(18):3932-42 PMID: 9127
  43. Use of thin-layer chromatography in the separation of disaccharides resulting from digestion of chondroitin sulphates with chondroitinases.
    J Chromatogr. 1977 Jun 11;136(2):342-7 PMID: 885969
  44. Transforming growth factor-beta stimulates the expression of fibronectin and collagen and their incorporation into the extracellular matrix.
    J Biol Chem. 1986 Mar 25;261(9):4337-45 PMID: 3456347
  45. Heparan sulfate proteoglycans of human lung fibroblasts. Occurrence of distinct membrane, matrix and secreted forms.
    Eur J Biochem. 1986 Jul 15;158(2):351-9 PMID: 2942402
  46. Identification and synthesis of a recognition signal for the attachment of glycosaminoglycans to proteins.
    Proc Natl Acad Sci U S A. 1987 May;84(10):3194-8 PMID: 3472204
  47. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  48. Integral membrane heparan sulfate proteoglycans.
    FASEB J. 1993 Aug;7(11):1023-30 PMID: 8370471
  49. CD44 and its interaction with extracellular matrix.
    Adv Immunol. 1993;54:271-335 PMID: 8379464
  50. Cell surface CD44-related chondroitin sulfate proteoglycan is required for transforming growth factor-beta-stimulated mouse melanoma cell motility and invasive behavior on type I collagen.
    J Cell Sci. 1993 Jun;105 ( Pt 2):501-11 PMID: 7691842
  51. The coordinated regulation of heparan sulfate, syndecans and cell behavior.
    Curr Opin Cell Biol. 1993 Oct;5(5):844-53 PMID: 8240828
  52. Cerebroglycan: an integral membrane heparan sulfate proteoglycan that is unique to the developing nervous system and expressed specifically during neuronal differentiation.
    J Cell Biol. 1994 Jan;124(1-2):149-60 PMID: 8294498
  53. Core protein structure and sequence determine the site and presence of heparan sulfate and chondroitin sulfate on syndecan-1.
    J Biol Chem. 1994 Apr 22;269(16):12304-9 PMID: 8163535
  54. Amino acid determinants that drive heparan sulfate assembly in a proteoglycan.
    J Biol Chem. 1994 Jul 29;269(30):19295-9 PMID: 8034692
  55. Interaction of the small interstitial proteoglycans biglycan, decorin and fibromodulin with transforming growth factor beta.
    Biochem J. 1994 Sep 1;302 ( Pt 2):527-34 PMID: 8093006
  56. Members of the syndecan family of heparan sulfate proteoglycans are expressed in distinct cell-, tissue-, and development-specific patterns.
    Mol Biol Cell. 1994 Jul;5(7):797-805 PMID: 7812048
  57. Characterization of the class III collagen receptor, a phosphorylated, transmembrane glycoprotein expressed in nucleated human cells.
    J Biol Chem. 1988 Mar 25;263(9):4193-201 PMID: 2831221
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj3100073
Published
1995-08-15
Pages
73-81
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1135856
Subset
IM
External Links
PubMed source DOI: 10.1042/bj3100073 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2