Home LiteratureArticle Details
PMID: 7544046 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Analysis of human immunodeficiency virus type 1 integrase mutants.

Virology ·Vol. 211 ·No. 1 ·1995-08-01 ·Pages 332-5

Ansari-Lari MA, Donehower LA, Gibbs RA

Abstract

The human immunodeficiency virus type-1 (HIV-1) integrase protein (IN) mediates the insertion of linear double-stranded viral DNA into the host genome. Mutations in IN can have different effects on the virus life cycle. In this study, Gag-Pol polyprotein processing, Tat synthesis, and viral replication were investigated in integrase-defective HIV-1 mutants. In the absence of IN synthesis, the Gag-Pol polyprotein stability, packaging, and/or processing was reduced. There was limited expression of Tat observed in IN mutants, but no viral replication.

MeSH Terms
Base Sequence Blotting, Western Cell Line DNA Nucleotidyltransferases/genetics,metabolism DNA Primers Defective Viruses/enzymology,genetics Gene Products, gag/biosynthesis Gene Products, pol/biosynthesis Genome, Viral HIV Reverse Transcriptase HIV-1/enzymology,genetics Humans Integrases Molecular Sequence Data Point Mutation Polymerase Chain Reaction RNA-Directed DNA Polymerase/metabolism Transfection Viral Proteins/biosynthesis,genetics,isolation & purification
Chemicals
DNA Primers Gene Products, gag Gene Products, pol Viral Proteins DNA Nucleotidyltransferases Integrases HIV Reverse Transcriptase RNA-Directed DNA Polymerase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ansari-Lari M A
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
Donehower L A
Gibbs R A
Article Info
Journal
Virology
Abbr.
Virology
ISSN
0042-6822
DOI
10.1006/viro.1995.1412
Published
1995-08-01
Pages
332-5
Language
English
Region
United States
NLM ID
0110674
Subset
IM
Grants
NIAID NIH HHS · 1P30AI36211 · United States
NIAID NIH HHS · UO1AI30243 · United States
Corrections
ErratumIn
PMID: 7491794
External Links
PubMed source DOI: 10.1006/viro.1995.1412 View journal details
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