Identification of the domain which determines the g,m serotype of the flagellin of Salmonella enteritidis.

Journal of bacteriology ·Vol. 177 ·No. 6 ·1995-03-00 ·Pages 1610-3

van Asten AJ, Zwaagstra KA, Baay MF, Kusters JG, Huis in't Veld JH, van der Zeijst BA

Abstract

Clones expressing fragments of the flagellin protein of Salmonella enteritidis were constructed and screened with a g,m-specific monoclonal antibody. Results showed that the g,m epitope is localized between amino acids 258 and 348 of the flagellin. The fliC gene, encoding the flagellin of S. enteritidis, was proven to be the only flagellin gene present in S. enteritidis.

MeSH Terms

Amino Acid Sequence Antigens, Bacterial/genetics,immunology Base Sequence Cloning, Molecular Epitope Mapping Epitopes/genetics,immunology Flagellin/genetics,immunology Molecular Sequence Data Peptide Fragments/genetics,immunology Salmonella enteritidis/genetics,immunology Sequence Homology, Amino Acid Serotyping

Chemicals

Antigens, Bacterial Epitopes Peptide Fragments Flagellin

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

van Asten A J

Department of Bacteriology, University of Utrecht, The Netherlands.

Zwaagstra K A

Baay M F

Kusters J G

Huis in't Veld J H

van der Zeijst B A

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24 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.177.6.1610-1613.1995

Published

1995-03-00

Pages

1610-3

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC176779

Subset

Databases

GENBANK

U12963

External Links

PubMed source DOI: 10.1128/jb.177.6.1610-1613.1995 PMC Full Text View journal details

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Identification of the domain which determines the g,m serotype of the flagellin of Salmonella enteritidis.

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