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PMID: 752725 Published · ppublish English Journal Article

Metabolic stimulation of polymorphonuclear leucocytes: effects of tetravalent and divalent concanavalin A.

The Journal of membrane biology ·Vol. 44 ·No. 3-4 ·1978-12-29 ·Pages 321-30

Romeo D, Zabucchi G, Berton G, Schneider C

Abstract

Polymorphonuclear leucocytes (PMNL) undergo a marked activation of their oxidative metabolism upon interaction with surface-reactive soluble stimuli as well as with phagocytosable objects. To get some insight into the mechanism of this stimulation, we have compared the stimulatory activity of the tetravalent lectin concanavalin A (Con A) with that of the divalent derivative succinyl-Con A (S-Con A). Both lectins bind to the PMNL surface to the same extent. S-Con A, however, is much less efficient in stimulating the PMNL metabolism. When S-Con A-treated PMNL are further reacted with antiserum to Con A, a potentiation of the metabolic stimulation is observed. Normal serum or addition to PMNL of antiserum to Con A in the absence of lectin has no effect. Furthermore, if S-Con A is displaced from its receptors on the cell membrane with alpha-methyl mannopyranoside, the addition of antiserum fails to cause a respiratory stimulation. These results suggest that the initial triggering of the metabolic stimulation of PMNL is in part accomplished through cross-linkage of membrane constituents.

MeSH Terms
Antibodies Antigen-Antibody Reactions Concanavalin A/analogs & derivatives,immunology,pharmacology Humans Neutrophils/metabolism Oxidation-Reduction Receptors, Concanavalin A/metabolism Receptors, Drug/metabolism
Chemicals
Antibodies Receptors, Concanavalin A Receptors, Drug Concanavalin A
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Romeo D
Zabucchi G
Berton G
Schneider C
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33 references, click to expand
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01944227
Published
1978-12-29
Pages
321-30
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01944227 View journal details
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