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PMID: 7525849 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sulfation-dependent recognition of high endothelial venules (HEV)-ligands by L-selectin and MECA 79, and adhesion-blocking monoclonal antibody.

The Journal of experimental medicine ·Vol. 180 ·No. 6 ·1994-12-01 ·Pages 2219-26

Hemmerich S, Butcher EC, Rosen SD

Abstract

L-selectin is a lectin-like receptor that mediates the attachment of lymphocytes to high endothelial venules (HEV) of lymph nodes during the process of lymphocyte recirculation. Two sulfated, mucin-like glycoproteins known as Sgp50/GlyCAM-1 and Sgp90/CD34 have previously been identified as HEV-associated ligands for L-selectin. These proteins were originally detected with an L-selectin/Ig chimera called LEC-IgG. GlyCAM-1 and CD34 are also recognized by an antiperipheral node addressin (PNAd) mAb called MECA 79, which blocks L-selectin-dependent adhesion and selectively stains lymph node HEV. The present study compares the requirements for the binding of MECA 79 and LEC-IgG to HEV-ligands. Whereas desialylation of GlyCAM-1 and CD34 drastically reduced binding to LEC-IgG, this treatment enhanced the binding of GlyCAM-1 to MECA 79. In contrast, the binding of both MECA 79 and LEC-IgG to GlyCAM-1 and CD34 was greatly decreased when the sulfation of these ligands was reduced with chlorate, a metabolic inhibitor of sulfation. Because MECA 79 stains HEV-like vessels at various sites of inflammation, recognition by L-selectin of ligands outside of secondary lymphoid organs may depend on sulfation. In addition to their reactivity with GlyCAM-1 and CD34, both MECA 79 and LEC-IgG recognize an independent molecule of approximately 200 kD in a sulfate-dependent manner. Thus, this molecule, which we designate Sgp200, is an additional ligand for L-selectin.

MeSH Terms
Amino Acid Sequence Animals Antibodies Antibodies, Monoclonal/pharmacology Antigens, CD/immunology,physiology Antigens, CD34 Binding Sites Cell Adhesion/drug effects,immunology,physiology Cell Adhesion Molecules/pharmacology Cells, Cultured Endothelium, Vascular/physiology Galactose/metabolism L-Selectin Lymph Nodes/blood supply Lymphocytes/physiology Mice Mice, Inbred ICR Molecular Sequence Data Mucins/immunology,physiology Peptides/chemical synthesis,immunology Recombinant Fusion Proteins/pharmacology Sulfates/metabolism Sulfur Radioisotopes Tritium
Chemicals
Antibodies Antibodies, Monoclonal Antigens, CD Antigens, CD34 Cell Adhesion Molecules Mucins Peptides Recombinant Fusion Proteins Sulfates Sulfur Radioisotopes Tritium L-Selectin sulfated glycoprotein p50 Galactose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hemmerich S
Department of Anatomy, University of California, San Francisco 94143-0452.
Butcher E C
Rosen S D
References (57)
57 references, click to expand
  1. Myelin localization of a central nervous system ligand for L-selectin.
    J Neuroimmunol. 1994 Sep;53(2):133-41 PMID: 7520918
  2. Expression of homing and adhesion molecules in infiltrated islets of Langerhans and salivary glands of nonobese diabetic mice.
    J Immunol. 1994 Jun 15;152(12):5969-78 PMID: 8207221
  3. Lymphocyte homing and leukocyte rolling and migration are impaired in L-selectin-deficient mice.
    Immunity. 1994 Jul;1(4):247-60 PMID: 7534203
  4. Molecules controlling lymphocyte-endothelial interactions in lymph nodes are produced in vessels of inflamed synovium.
    Ann Rheum Dis. 1987 Dec;46(12):924-8 PMID: 3426301
  5. Two distinct classes of carbohydrate-recognition domains in animal lectins.
    J Biol Chem. 1988 Jul 15;263(20):9557-60 PMID: 3290208
  6. Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes.
    J Cell Biol. 1988 Nov;107(5):1853-62 PMID: 2460470
  7. Intravenously injected sialidase inactivates attachment sites for lymphocytes on high endothelial venules.
    J Immunol. 1989 Mar 15;142(6):1895-902 PMID: 2921520
  8. A homing receptor-IgG chimera as a probe for adhesive ligands of lymph node high endothelial venules.
    J Cell Biol. 1990 Jun;110(6):2221-9 PMID: 2190992
  9. Direct demonstration of the lectin activity of gp90MEL, a lymphocyte homing receptor.
    J Cell Biol. 1990 Sep;111(3):1225-32 PMID: 2202735
  10. Identification of a carbohydrate-based endothelial ligand for a lymphocyte homing receptor.
    J Cell Biol. 1991 Jun;113(5):1213-21 PMID: 2040648
  11. The human peripheral lymph node vascular addressin is a ligand for LECAM-1, the peripheral lymph node homing receptor.
    J Cell Biol. 1991 Jul;114(2):343-9 PMID: 1712790
  12. Molecular features of CD34: a hemopoietic progenitor cell-associated molecule.
    Leukemia. 1992;6 Suppl 1:31-6 PMID: 1372379
  13. Comparison of L-selectin and E-selectin ligand specificities: the L-selectin can bind the E-selectin ligands sialyl Le(x) and sialyl Le(a).
    Biochem Biophys Res Commun. 1992 Apr 30;184(2):1048-55 PMID: 1374233
  14. The three members of the selectin receptor family recognize a common carbohydrate epitope, the sialyl Lewis(x) oligosaccharide.
    J Cell Biol. 1992 May;117(4):895-902 PMID: 1374413
  15. P-selectin and E-selectin. Distinct but overlapping leukocyte ligand specificities.
    J Biol Chem. 1992 Jun 5;267(16):11104-10 PMID: 1375936
  16. An endothelial ligand for L-selectin is a novel mucin-like molecule.
    Cell. 1992 Jun 12;69(6):927-38 PMID: 1376638
  17. Altered patterns of T cell migration through lymph nodes and skin following antigen challenge.
    Eur J Immunol. 1992 Sep;22(9):2205-10 PMID: 1381308
  18. Identification of a soluble form of a ligand for the lymphocyte homing receptor.
    J Exp Med. 1992 Nov 1;176(5):1415-9 PMID: 1383387
  19. High affinity binding of the leucocyte adhesion molecule L-selectin to 3'-sulphated-Le(a) and -Le(x) oligosaccharides and the predominance of sulphate in this interaction demonstrated by binding studies with a series of lipid-linked oligosaccharides.
    Biochem Biophys Res Commun. 1992 Oct 15;188(1):244-51 PMID: 1384480
  20. Further characterization of the interaction between L-selectin and its endothelial ligands.
    Glycobiology. 1992 Aug;2(4):373-81 PMID: 1384820
  21. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  22. Migration of lymphocytes across specialized vascular endothelium. V. Production of a sulphated macromolecule by high endothelial cells in lymph nodes.
    J Cell Sci. 1982 Oct;57:277-92 PMID: 7153260
  23. Possible role for cell-surface carbohydrate-binding molecules in lymphocyte recirculation.
    J Cell Biol. 1983 Mar;96(3):722-9 PMID: 6833380
  24. Fractionation of L-fucose-containing oligosaccharides on immobilized Aleuria aurantia lectin.
    J Biol Chem. 1985 Apr 25;260(8):4688-93 PMID: 3988732
  25. Involvement of sialic acid on endothelial cells in organ-specific lymphocyte recirculation.
    Science. 1985 May 24;228(4702):1005-7 PMID: 4001928
  26. The regulation of lymphocyte traffic.
    Curr Top Microbiol Immunol. 1986;128:85-122 PMID: 3533449
  27. Expression of a tumor necrosis factor alpha transgene in murine pancreatic beta cells results in severe and permanent insulitis without evolution towards diabetes.
    J Exp Med. 1992 Dec 1;176(6):1719-31 PMID: 1460428
  28. Selective expression of sialyl-Lewis x and Lewis a epitopes, putative ligands for L-selectin, on peripheral lymph-node high endothelial venules.
    Am J Pathol. 1992 Dec;141(6):1259-64 PMID: 1281614
  29. Sulfated glycolipids are ligands for a lymphocyte homing receptor, L-selectin (LECAM-1), Binding epitope in sulfated sugar chain.
    Biochem Biophys Res Commun. 1993 Jan 29;190(2):426-34 PMID: 7678958
  30. Sulphation requirement for GlyCAM-1, an endothelial ligand for L-selectin.
    Nature. 1993 Feb 11;361(6412):555-7 PMID: 7679207
  31. Selectins.
    J Clin Invest. 1993 Feb;91(2):379-87 PMID: 7679406
  32. The HNK-1 reactive sulfoglucuronyl glycolipids are ligands for L-selectin and P-selectin but not E-selectin.
    Proc Natl Acad Sci U S A. 1993 Feb 15;90(4):1359-63 PMID: 7679503
  33. Molecular mechanisms underlying lymphocyte recirculation. III. Characterization of the LECAM-1 (L-selectin)-dependent adhesion pathway in rats.
    J Immunol. 1993 Mar 1;150(5):1735-45 PMID: 7679693
  34. Leukocyte-endothelial cell recognition: evidence of a common molecular mechanism shared by neutrophils, lymphocytes, and other leukocytes.
    J Immunol. 1987 Jun 15;138(12):4313-21 PMID: 3584977
  35. Properties of a sialic acid-specific lectin from the slug Limax flavus.
    Methods Enzymol. 1987;138:527-36 PMID: 3600346
  36. MAdCAM-1 has homology to immunoglobulin and mucin-like adhesion receptors and to IgA1.
    Nature. 1993 Jun 3;363(6428):461-4 PMID: 8502297
  37. Specific expression of a complex sialyl Lewis X antigen on high endothelial venules of human lymph nodes: possible candidate for L-selectin ligand.
    Biochem Biophys Res Commun. 1993 May 28;193(1):337-47 PMID: 7684905
  38. Leukocyte extravasation into the pancreatic tissue in transgenic mice expressing interleukin 10 in the islets of Langerhans.
    J Exp Med. 1993 Jul 1;178(1):175-85 PMID: 8100268
  39. Calcium-dependent heparin-like ligands for L-selectin in nonlymphoid endothelial cells.
    Science. 1993 Jul 23;261(5120):480-3 PMID: 7687382
  40. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1.
    Cell. 1993 Jul 16;74(1):185-95 PMID: 7687523
  41. Direct demonstration of heterogeneous, sulfated O-linked carbohydrate chains on an endothelial ligand for L-selectin.
    Glycoconj J. 1993 Feb;10(1):34-9 PMID: 7689376
  42. L- and E-selectin can recognize the same naturally occurring ligands on high endothelial venules.
    J Immunol. 1993 Sep 15;151(6):3252-60 PMID: 7690798
  43. Binding of L-selectin to the vascular sialomucin CD34.
    Science. 1993 Oct 15;262(5132):436-8 PMID: 7692600
  44. Vascular addressins are induced on islet vessels during insulitis in nonobese diabetic mice and are involved in lymphoid cell binding to islet endothelium.
    J Clin Invest. 1993 Nov;92(5):2509-15 PMID: 7693764
  45. The human peripheral lymph node vascular addressin. An inducible endothelial antigen involved in lymphocyte homing.
    Am J Pathol. 1993 Dec;143(6):1688-98 PMID: 8256856
  46. L-selectin-mediated lymphocyte rolling on MAdCAM-1.
    Nature. 1993 Dec 16;366(6456):695-8 PMID: 7505053
  47. Expression cloning of a functional glycoprotein ligand for P-selectin.
    Cell. 1993 Dec 17;75(6):1179-86 PMID: 7505206
  48. Developmental switches in the immune system.
    Cell. 1994 Jan 28;76(2):207-18 PMID: 8293459
  49. Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm.
    Cell. 1994 Jan 28;76(2):301-14 PMID: 7507411
  50. Aberrant binding of lamina propria lymphocytes to vascular endothelium in inflammatory bowel diseases.
    Gastroenterology. 1994 Mar;106(3):596-605 PMID: 8119529
  51. Role of alpha 4-integrins in lymphocyte homing to mucosal tissues in vivo.
    J Immunol. 1994 Apr 1;152(7):3282-93 PMID: 7511642
  52. Induction of vascular addressins and adhesion molecules in the pancreas of IFN-gamma transgenic mice.
    J Immunol. 1994 May 1;152(9):4597-603 PMID: 7512601
  53. Identification of the sulfated monosaccharides of GlyCAM-1, an endothelial-derived ligand for L-selectin.
    Biochemistry. 1994 Apr 26;33(16):4820-9 PMID: 7512827
  54. 6'-sulfated sialyl Lewis x is a major capping group of GlyCAM-1.
    Biochemistry. 1994 Apr 26;33(16):4830-5 PMID: 8161542
  55. Monocyte rolling, arrest and spreading on IL-4-activated vascular endothelium under flow is mediated via sequential action of L-selectin, beta 1-integrins, and beta 2-integrins.
    J Cell Biol. 1994 Jun;125(6):1417-27 PMID: 7515891
  56. Selectins: interpreters of cell-specific carbohydrate information during inflammation.
    Science. 1992 Nov 6;258(5084):964-9 PMID: 1439808
  57. The selectins and their ligands.
    Curr Opin Cell Biol. 1994 Oct;6(5):663-73 PMID: 7530461
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.180.6.2219
Published
1994-12-01
Pages
2219-26
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2191797
Subset
IM
Grants
NIGMS NIH HHS · GM-23547 · United States
NIGMS NIH HHS · GM-37734 · United States
External Links
PubMed source DOI: 10.1084/jem.180.6.2219 PMC Full Text View journal details
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