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PMID: 7524097 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

ard-1: a human gene that reverses the effects of temperature-sensitive and deletion mutations in the Escherichia coli rne gene and encodes an activity producing RNase E-like cleavages.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 22 ·1994-10-25 ·Pages 10591-5

Wang M, Cohen SN

Abstract

The Escherichia coli rne gene affects a variety of bacterial functions, including the activity of RNase E. We report the existence of a human gene (ard-1, for activator of RNA decay) that complements temperature-sensitive and deletion mutations of rne in E. coli, allowing growth of rne-defective cells, correcting abnormal cell shape, activating chemical decay of bulk mRNA, and producing site-specific cleavages characteristic of RNase E in vivo and in vitro. ard-1 encodes a highly basic 13.3-kDa proline-rich peptide that has features in common with Rne and also with eukaryotic proteins implicated in RNA binding and macromolecular transport.

Related Genes
ams ard-1 hmp1 rne
MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern Chromosomes, Bacterial DNA, Complementary/metabolism Endoribonucleases/genetics,metabolism Escherichia coli/genetics,growth & development,metabolism Genes, Bacterial Humans Kinetics Molecular Sequence Data Mutagenesis Open Reading Frames Phosphoprotein Phosphatases Plasmids RNA, Bacterial/metabolism RNA, Messenger/metabolism RNA-Binding Proteins Restriction Mapping Sequence Deletion Temperature
Chemicals
DNA, Complementary RNA, Bacterial RNA, Messenger RNA-Binding Proteins Endoribonucleases Phosphoprotein Phosphatases PPP1R8 protein, human ribonuclease E
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wang M
Department of Genetics, Stanford University School of Medicine, CA 94305.
Cohen S N
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.22.10591
Published
1994-10-25
Pages
10591-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC45067
Subset
IM
Grants
NEI NIH HHS · EYO7106 · United States
NIGMS NIH HHS · GM27241 · United States
Databases
GENBANK
U14575
External Links
PubMed source DOI: 10.1073/pnas.91.22.10591 PMC Full Text View journal details
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