V3 region polymorphisms in HIV-1 from Brazil: prevalence of subtype B strains divergent from North American/European prototype and detection of subtype F.

AIDS research and human retroviruses ·Vol. 10 ·No. 5 ·1994-05-00 ·Pages 569-76

Morgado MG, Sabino EC, Shpaer EG, Bongertz V, Brigido L, Guimaraes MD, Castilho EA, Galvão-Castro B, Mullins JI, Hendry RM

Abstract

Viral DNA sequences were determined over the V3 region of env from 28 infected individuals living in the high HIV-1 prevalence Brazilian cities of Rio de Janeiro and São Paulo. Twenty-six belonged to envelope sequence subtype B, prevalent in North America and Europe, and one was classified as subtype F, found recently in Brazil and in Romania (one appeared to be a B/F recombinant). Octameric sequences at the tip of the subtype B V3 loops were variable and distinct from those prevalent in North America and Europe. The GPGR motif, prevalent in North American/European strains, was found in only 8 (28.5%) sequences, whereas GWGR was found in 12 (43%) and novel sequences in 8 (28.5%). Brazilian subtype B sequences also diverged from the consensus North American/European strains over the remainder of the V3 loop. These results suggest that Brazilian HIV-1 B strains may have important antigenic differences from prototype subtype B strains currently being evaluated for use in HIV vaccines. These results should be taken into account for future vaccine programs in Brazil.

Related Genes

env

MeSH Terms

AIDS Vaccines/isolation & purification Amino Acid Sequence Base Sequence Brazil DNA Primers/genetics DNA, Viral/genetics Female Genes, env HIV Envelope Protein gp120/genetics HIV Infections/microbiology HIV-1/classification,genetics,isolation & purification Humans Male Molecular Sequence Data Peptide Fragments/genetics Phylogeny Polymerase Chain Reaction Polymorphism, Genetic RNA/genetics Sequence Homology, Amino Acid

Chemicals

AIDS Vaccines DNA Primers DNA, Viral HIV Envelope Protein gp120 HIV envelope protein gp120 (305-321) Peptide Fragments RNA primers RNA

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Morgado M G

Department of Immunology, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil.

Sabino E C

Shpaer E G

Bongertz V

Brigido L

Guimaraes M D

Castilho E A

Galvão-Castro B

Mullins J I

Hendry R M

Article Info

Journal

AIDS research and human retroviruses

Abbr.

AIDS Res Hum Retroviruses

ISSN

0889-2229

DOI

10.1089/aid.1994.10.569

Published

1994-05-00

Pages

569-76

Language

English

Region

United States

NLM ID

8709376

Subset

Databases

GENBANK

U00400, U00401, U00402, U00403, U00404, U00405, U00406, U00407, U00408, U00409, U00410, U00411, U00412, U00413, U00414, U00415, U00416, U00417, U00418, U00419, U00420, U00421, U00422, U00423, U00424, U00425, U00426, U00427

External Links

PubMed source DOI: 10.1089/aid.1994.10.569 View journal details

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V3 region polymorphisms in HIV-1 from Brazil: prevalence of subtype B strains divergent from North American/European prototype and detection of subtype F.

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