Abstract
cDNA clones encoding three additional serine carboxypeptidases (Ser-CPs) have been isolated from a gibberellic acid-induced barley aleurone cDNA library. The three deduced Ser-CPs belong to the two-chain subfamily of Ser-CPs; they are synthesized as precursors with a putative signal peptide, propeptide, and linker peptide between the A and B chains. Their identification provides the proof for the existence of more than three Ser-CPs in cereal grains, and, based on their sequences, they may exhibit new substrate specificities. The expression of these and of the three previously isolated Ser-CPs from barley grains (CP-MI, CP-MII, and CP-MIII) has been investigated by Northern and Western analysis and RNA PCR. CP-MII is the only Ser-CP to be expressed and accumulate in the developing grain and is stored in its active form in the mature grain. All six Ser-CPs are expressed de novo in the germinating grain, in the scutellum, and/or in the aleurone. Furthermore, at least CP-MI, CP-MII, and CP-MIII are secreted into the endosperm. In addition, all Ser-CPs (except CP-MI) are also expressed in the roots and shoots of the growing seedling. This enzyme family thus appears to be ubiquitous in the barley plant, which suggests that Ser-CPs play additional roles besides their participation in the mobilization of storage proteins.
MeSH Terms
Amino Acid Sequence
Base Sequence
Carboxypeptidases/biosynthesis,isolation & purification,metabolism
Cloning, Molecular
DNA Probes
Gene Expression Regulation, Enzymologic
Gene Library
Genes, Plant
Hordeum/enzymology,physiology
Isoenzymes/biosynthesis,isolation & purification,metabolism
Molecular Sequence Data
Polymerase Chain Reaction
RNA/biosynthesis,isolation & purification
Seeds/enzymology,physiology
Sequence Homology, Amino Acid
Substrate Specificity
Chemicals
DNA Probes
Isoenzymes
RNA
Carboxypeptidases
serine carboxypeptidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dal Degan F
Department of Physiology, Carlsberg Laboratory, Copenhagen Valby, Denmark.
Rocher A
Cameron-Mills V
von Wettstein D
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