Home LiteratureArticle Details
PMID: 7519125 Published · ppublish English Comparative Study Journal Article

Generation of specific anti-melanoma reactivity by stimulation of human tumor-infiltrating lymphocytes with MAGE-1 synthetic peptide.

Cancer immunology, immunotherapy : CII ·Vol. 39 ·No. 2 ·1994-08-00 ·Pages 105-16

Salgaller ML, Weber JS, Koenig S, Yannelli JR, Rosenberg SA

Abstract

The MAGE-1 gene encodes a tumor-specific antigen, MZ2-E, which is recognized by cloned, specific cytolytic T cells (CTL) derived from the peripheral blood of a patient with melanoma. We have produced a MAGE-1-specific CTL line derived from the tumor-infiltrating lymphocytes (TIL) of a melanoma patient by weekly restimulation with autologous EBV-B cells pulsed with the synthetic HLA-A1-restricted MAGE-1 epitope nonapeptide EADPTGHSY. The 1277.A TIL line grew in long-term culture in low-dose interleukin-2 (IL-2) and IL-4, and exhibited antigen-specific, MHC-class-I-restricted lysis of HLA-A1-bearing MAGE-1+ cell lines. Cytolysis of target cells pulsed with the synthetic MAGE-1 decapeptide KEADPTGHSY was superior to that of cells pulsed with the immunodominant nonapeptide. Single amino-acid or even side-chain substitutions in the immunodominant nonamer abrogated cytolysis. 1277.A TIL specifically secreted tumor necrosis factor alpha after co-incubation with HLA-A1-expressing MAGE-1+ cell lines or fresh tumor. These data suggest that tumor-antigen-specific, MHC-restricted CTL may be grown from TIL in the presence of synthetic epitope peptides and expanded for adoptive immunotherapy in melanoma patients.

Related Genes
MAGE-1
MeSH Terms
Amino Acid Sequence Antigens, Neoplasm/genetics,immunology,pharmacology B-Lymphocytes/immunology Base Sequence Cell Division/physiology Cell Transformation, Viral Cytokines/metabolism Cytotoxicity, Immunologic Epitopes Gene Expression HLA-A1 Antigen Herpesvirus 4, Human Humans Immunotherapy, Adoptive Interleukin-2/pharmacology Interleukin-4/pharmacology Lymphocyte Activation/drug effects Lymphocytes, Tumor-Infiltrating/cytology,drug effects,immunology Melanoma/blood,genetics,immunology Melanoma-Specific Antigens Molecular Sequence Data Neoplasm Proteins RNA, Neoplasm/analysis Sensitivity and Specificity Stimulation, Chemical T-Lymphocytes, Cytotoxic/cytology,drug effects,immunology
Chemicals
Antigens, Neoplasm Cytokines Epitopes HLA-A1 Antigen Interleukin-2 MAGEA1 protein, human Melanoma-Specific Antigens Neoplasm Proteins RNA, Neoplasm Interleukin-4
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Salgaller M L
Surgery Branch, National Cancer Institute, Bethesda, Maryland 20892.
Weber J S
Koenig S
Yannelli J R
Rosenberg S A
References (53)
53 references, click to expand
  1. Role of the major histocompatibility complex class I antigens in tumor growth and metastasis.
    Annu Rev Immunol. 1988;6:359-80 PMID: 3289570
  2. A nonimmunogenic sarcoma transduced with the cDNA for interferon gamma elicits CD8+ T cells against the wild-type tumor: correlation with antigen presentation capability.
    J Exp Med. 1992 Jun 1;175(6):1423-31 PMID: 1588273
  3. A nonapeptide encoded by human gene MAGE-1 is recognized on HLA-A1 by cytolytic T lymphocytes directed against tumor antigen MZ2-E.
    J Exp Med. 1992 Nov 1;176(5):1453-7 PMID: 1402688
  4. Structure of the gene of tum- transplantation antigen P198: a point mutation generates a new antigenic peptide.
    J Exp Med. 1990 Jul 1;172(1):35-45 PMID: 1694221
  5. Tumor antigens recognized by cytolytic T lymphocytes: present perspectives for specific immunotherapy.
    Int J Cancer. 1993 May 8;54(2):177-80 PMID: 8486420
  6. Tumor vaccines.
    Cancer Metastasis Rev. 1990 Jul;9(1):81-91 PMID: 2208570
  7. Antigen-driven T cell clones can proliferate in vivo, eradicate disseminated leukemia, and provide specific immunologic memory.
    J Immunol. 1987 Jun 1;138(11):4012-7 PMID: 3108381
  8. Toward a genetic analysis of tumor rejection antigens.
    Adv Cancer Res. 1992;58:177-210 PMID: 1546558
  9. Perspectives on the role of MHC antigens in normal and malignant cell development.
    Adv Cancer Res. 1989;53:181-245 PMID: 2678947
  10. A gene encoding an antigen recognized by cytolytic T lymphocytes on a human melanoma.
    Science. 1991 Dec 13;254(5038):1643-7 PMID: 1840703
  11. Dendritic cells pulsed with protein antigens in vitro can prime antigen-specific, MHC-restricted T cells in situ.
    J Exp Med. 1990 Aug 1;172(2):631-40 PMID: 2373994
  12. The role of HLA class I antigens in recognition of melanoma cells by tumor-specific cytotoxic T lymphocytes. Evidence for shared tumor antigens.
    J Immunol. 1989 May 1;142(9):3329-35 PMID: 2785141
  13. The epitopes of influenza nucleoprotein recognized by cytotoxic T lymphocytes can be defined with short synthetic peptides.
    Cell. 1986 Mar 28;44(6):959-68 PMID: 2420472
  14. Identification of human cancers deficient in antigen processing.
    J Exp Med. 1993 Feb 1;177(2):265-72 PMID: 8426105
  15. CD4+ T cell clones specific for the human p97 melanoma-associated antigen can eradicate pulmonary metastases from a murine tumor expressing the p97 antigen.
    J Immunol. 1991 May 1;146(9):3235-41 PMID: 1707934
  16. Interferon gamma and tumor necrosis factor have a role in tumor regressions mediated by murine CD8+ tumor-infiltrating lymphocytes.
    J Exp Med. 1991 Mar 1;173(3):647-58 PMID: 1900079
  17. Identification of two cytotoxic T lymphocyte-recognized epitopes in the Ras protein.
    J Exp Med. 1993 May 1;177(5):1493-8 PMID: 7683038
  18. Presentation of soluble antigen to human T cells by products of multiple HLA-linked loci: analysis of antigen presentation by a panel of cloned, autologous, HLA-mutant Epstein-Barr virus-transformed lymphoblastoid cell lines.
    Hum Immunol. 1987 Jan;18(1):75-91 PMID: 3542914
  19. Demonstration of resistance against methylcholanthrene-induced sarcomas in the primary autochthonous host.
    Cancer Res. 1960 Dec;20:1561-72 PMID: 13756652
  20. Expansion of human tumor infiltrating lymphocytes for use in immunotherapy trials.
    J Immunol Methods. 1987 Aug 24;102(1):127-41 PMID: 3305708
  21. The tyrosinase gene codes for an antigen recognized by autologous cytolytic T lymphocytes on HLA-A2 melanomas.
    J Exp Med. 1993 Aug 1;178(2):489-95 PMID: 8340755
  22. Role of IL-6, IL-2, and IL-4 in the in vitro induction of cytotoxic T cells.
    J Immunol. 1992 Nov 15;149(10):3316-20 PMID: 1431108
  23. Expression of the MAGE-1 tumor antigen is up-regulated by the demethylating agent 5-aza-2'-deoxycytidine.
    Cancer Res. 1994 Apr 1;54(7):1766-71 PMID: 7511051
  24. Shared human melanoma antigens. Recognition by tumor-infiltrating lymphocytes in HLA-A2.1-transfected melanomas.
    J Immunol. 1992 Jan 15;148(2):638-43 PMID: 1729379
  25. Antigen presentation by human B cells: T cell proliferation induced by Epstein Barr virus B lymphoblastoid cells.
    J Immunol. 1982 Oct;129(4):1446-50 PMID: 6286767
  26. Adoptive immunotherapy with peripheral blood lymphocytes cocultured in vitro with autologous tumor cells and interleukin-2.
    Cancer Immunol Immunother. 1993 Aug;37(3):175-80 PMID: 8392912
  27. Antigen presentation. Naturally processed peptides.
    Nature. 1990 Nov 15;348(6298):195-7 PMID: 2172837
  28. A new approach to the adoptive immunotherapy of cancer with tumor-infiltrating lymphocytes.
    Science. 1986 Sep 19;233(4770):1318-21 PMID: 3489291
  29. Vaccination with cytotoxic T lymphocyte epitope-containing peptide protects against a tumor induced by human papillomavirus type 16-transformed cells.
    Eur J Immunol. 1993 Sep;23(9):2242-9 PMID: 7690326
  30. Fine specificity of cytotoxic T lymphocytes primed in vivo either with virus or synthetic lipopeptide vaccine or primed in vitro with peptide.
    J Exp Med. 1991 Dec 1;174(6):1665-8 PMID: 1744592
  31. Restoration of viral immunity in immunodeficient humans by the adoptive transfer of T cell clones.
    Science. 1992 Jul 10;257(5067):238-41 PMID: 1352912
  32. Use of tumor-infiltrating lymphocytes and interleukin-2 in the immunotherapy of patients with metastatic melanoma. A preliminary report.
    N Engl J Med. 1988 Dec 22;319(25):1676-80 PMID: 3264384
  33. Melanoma cells and normal melanocytes share antigens recognized by HLA-A2-restricted cytotoxic T cell clones from melanoma patients.
    J Exp Med. 1993 Apr 1;177(4):989-98 PMID: 8459226
  34. Differential expression of MAGE-1, -2, and -3 messenger RNA in transformed and normal human cell lines.
    Cancer Res. 1993 Jan 1;53(1):5-8 PMID: 8416750
  35. Unique tumor-specific antigens.
    Annu Rev Immunol. 1988;6:465-83 PMID: 3289573
  36. Precursor frequency analysis of human cytolytic T lymphocytes directed against autologous melanoma cells.
    Int J Cancer. 1992 Jan 21;50(2):289-97 PMID: 1730522
  37. Specific release of cytokines by lymphocytes infiltrating human melanomas in response to shared melanoma antigens.
    J Immunother Emphasis Tumor Immunol. 1993 Jan;13(1):18-30 PMID: 8435428
  38. Structure of the gene of tum- transplantation antigen P91A: the mutated exon encodes a peptide recognized with Ld by cytolytic T cells.
    Cell. 1989 Jul 28;58(2):293-303 PMID: 2568889
  39. The gene coding for a major tumor rejection antigen of tumor P815 is identical to the normal gene of syngeneic DBA/2 mice.
    J Exp Med. 1991 Jun 1;173(6):1373-84 PMID: 1903428
  40. Transfection and expression of a gene coding for a human melanoma antigen recognized by autologous cytolytic T lymphocytes.
    Immunogenetics. 1992;35(3):145-52 PMID: 1537606
  41. Antigen presentation by B cells and its significance in T-B interactions.
    Adv Immunol. 1986;39:51-94 PMID: 3538822
  42. Overlapping epitopes that are recognized by CD8+ HLA class I-restricted and CD4+ class II-restricted cytotoxic T lymphocytes are contained within an influenza nucleoprotein peptide.
    J Immunol. 1992 Feb 1;148(3):894-9 PMID: 1370522
  43. Presentation of three different viral peptides, HTLV-1 Tax, HCMV gB, and influenza virus M1, is determined by common structural features of the HLA-A2.1 molecule.
    J Immunol. 1992 Jul 1;149(1):214-21 PMID: 1607654
  44. Immunization with recombinant BCG-SIV elicits SIV-specific cytotoxic T lymphocytes in rhesus monkeys.
    J Immunol. 1993 Apr 1;150(7):3101-7 PMID: 8454878
  45. A progress report on the treatment of 157 patients with advanced cancer using lymphokine-activated killer cells and interleukin-2 or high-dose interleukin-2 alone.
    N Engl J Med. 1987 Apr 9;316(15):889-97 PMID: 3493432
  46. Human gene MAGE-1, which codes for a tumor-rejection antigen, is expressed by some breast tumors.
    Int J Cancer. 1992 Nov 11;52(5):839-41 PMID: 1428237
  47. Antigen recognition by class I-restricted T lymphocytes.
    Annu Rev Immunol. 1989;7:601-24 PMID: 2469442
  48. Specific release of granulocyte-macrophage colony-stimulating factor, tumor necrosis factor-alpha, and IFN-gamma by human tumor-infiltrating lymphocytes after autologous tumor stimulation.
    J Immunol. 1991 May 15;146(10):3674-81 PMID: 1902860
  49. Antigen-driven long term-cultured T cells proliferate in vivo, distribute widely, mediate specific tumor therapy, and persist long-term as functional memory T cells.
    J Exp Med. 1986 May 1;163(5):1100-12 PMID: 3084700
  50. Tumor-specific cytolysis by lymphocytes infiltrating human melanomas.
    J Immunol. 1989 May 15;142(10):3714-25 PMID: 2785562
  51. Clonal analysis of the cytolytic T-cell response to human tumors.
    Immunol Today. 1987;8(12):385-9 PMID: 25291274
  52. Immunogenic (tum-) variants of mouse tumor P815: cloning of the gene of tum- antigen P91A and identification of the tum- mutation.
    Proc Natl Acad Sci U S A. 1988 Apr;85(7):2274-8 PMID: 3127830
  53. A mutant p53 tumor suppressor protein is a target for peptide-induced CD8+ cytotoxic T-cells.
    Cancer Res. 1993 Jul 15;53(14):3257-61 PMID: 7686815
Article Info
Journal
Cancer immunology, immunotherapy : CII
Abbr.
Cancer Immunol Immunother
ISSN
0340-7004
DOI
10.1007/BF01525316
Published
1994-08-00
Pages
105-16
Language
English
Region
Germany
NLM ID
8605732
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01525316 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2