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PMID: 7514684 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Cross-neutralizing activity against divergent human immunodeficiency virus type 1 isolates induced by the gp41 sequence ELDKWAS.

Journal of virology ·Vol. 68 ·No. 6 ·1994-06-00 ·Pages 4031-4

Muster T, Guinea R, Trkola A, Purtscher M, Klima A, Steindl F, Palese P, Katinger H

Abstract

Previously we identified the highly conserved amino acids Glu-Leu-Asp-Lys-Trp-Ala (ELDKWA) on the ecto-domain of gp41 as the epitope of a neutralizing monoclonal antibody (2F5) directed against human immunodeficiency virus type 1. In the present study, the sequence defining the epitope was introduced into the loop of antigenic site B of the influenza virus hemagglutinin. The resulting chimeric virus was able to elicit ELDKWA-specific immunoglobulins G and A in antisera of mice. Moreover, the distantly related human immunodeficiency virus type 1 isolates MN, RF, and IIIB were neutralized by these antisera. These data suggest that this conserved B-cell epitope is a promising candidate for inclusion in a vaccine against AIDS. The results also show that influenza virus can be used to effectively present the antigenic structure of this B-cell epitope.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Monoclonal B-Lymphocytes/immunology Base Sequence Chimera/genetics,immunology Conserved Sequence Cross Reactions DNA Primers/genetics DNA, Viral/genetics Epitopes/genetics HIV Antibodies HIV Antigens/genetics HIV Envelope Protein gp41/genetics,immunology HIV-1/genetics,immunology Humans Mice Molecular Sequence Data Neutralization Tests
Chemicals
Antibodies, Monoclonal DNA Primers DNA, Viral Epitopes HIV Antibodies HIV Antigens HIV Envelope Protein gp41
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Muster T
Institut für Angewandte Mikrobiologie, Universität für Bodenkultur, Vienna, Austria.
Guinea R
Trkola A
Purtscher M
Klima A
Steindl F
Palese P
Katinger H
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.68.6.4031-4034.1994
Published
1994-06-00
Pages
4031-4
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC236911
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.68.6.4031-4034.1994 PMC Full Text View journal details
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