Ctk: a protein-tyrosine kinase related to Csk that defines an enzyme family.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 7 ·1994-03-29 ·Pages 2597-601

Klages S, Adam D, Class K, Fargnoli J, Bolen JB, Penhallow RC

Abstract

We used the polymerase chain reaction with degenerate oligonucleotide primers to search for Csk-related kinases. A cDNA coding for a Csk-like protein-tyrosine kinase was cloned from mouse brain and was designated ctk, for csk-type protein-tyrosine kinase. The 1.9-kb ctk mRNA was found to be expressed predominantly in brain and capable of encoding a 52-kDa protein-tyrosine kinase. The amino acid sequence of Ctk was found to possess 53% identity with mouse Csk, shared all the predicted structural features of Csk, and was capable of phosphorylating the carboxyl-terminal conserved tyrosine of Src family members. Our results thereby indicate that ctk represents a gene that defines a family of structurally and functionally related Csk-like protein-tyrosine kinases.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Brain/enzymology CSK Tyrosine-Protein Kinase Cloning, Molecular Mice Molecular Sequence Data Phosphorylation Protein-Tyrosine Kinases/classification,genetics Proto-Oncogene Proteins pp60(c-src)/metabolism Sequence Homology, Amino Acid Substrate Specificity Tissue Distribution src-Family Kinases

Chemicals

Matk protein, mouse Protein-Tyrosine Kinases CSK Tyrosine-Protein Kinase Proto-Oncogene Proteins pp60(c-src) src-Family Kinases

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Klages S

Department of Molecular Biology, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, NJ 08543.

Adam D

Class K

Fargnoli J

Bolen J B

Penhallow R C

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.91.7.2597

Published

1994-03-29

Pages

2597-601

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC43416

Subset

Databases

GENBANK

U05210, U05247

External Links

PubMed source DOI: 10.1073/pnas.91.7.2597 PMC Full Text View journal details

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Ctk: a protein-tyrosine kinase related to Csk that defines an enzyme family.

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