Calcium permeability of nicotinic acetylcholine receptor channels in bovine adrenal chromaffin cells.

Pflugers Archiv : European journal of physiology ·Vol. 425 ·No. 5-6 ·1993-12-00 ·Pages 511-7

Zhou Z, Neher E

Abstract

The fractional contribution of Ca to current flow through neuronal-type nicotinic acetylcholine receptor channels was determined by quantitative fluorescence microfluorimetry using fura-2. The method, which has been applied already to several types of cells and channels is described in detail here. At -70 mV and 2 mM external Ca concentration it was found that Ca contributes 2.5% to the net current. The fractional contribution was found to be voltage dependent, increasing at negative potentials e-fold for a 110 mV potential difference. Total non-specific cation current was found to have a bell-shaped dependence on external Ca concentration peaking at 2 mM.

MeSH Terms

Adrenal Glands/cytology,metabolism Animals Calcium/metabolism,pharmacology,physiology Calcium Channels/physiology Cattle Chromaffin System/cytology,metabolism Electrophysiology Fura-2 Ion Channel Gating Ion Channels/metabolism Permeability Receptors, Nicotinic/metabolism

Chemicals

Calcium Channels Ion Channels Receptors, Nicotinic Calcium Fura-2

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Zhou Z

Department of Medicine, Jewish Hospital of St. Louis, MO 63110.

Neher E

References (14)

14 references, click to expand

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Article Info

Journal

Pflugers Archiv : European journal of physiology

Abbr.

Pflugers Arch

ISSN

0031-6768

DOI

10.1007/BF00374879

Published

1993-12-00

Pages

511-7

Language

English

Region

Germany

NLM ID

0154720

Subset

External Links

PubMed source DOI: 10.1007/BF00374879 View journal details

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Calcium permeability of nicotinic acetylcholine receptor channels in bovine adrenal chromaffin cells.

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