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PMID: 7505827 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Pharmacology of the SV channel in the vacuolar membrane of Chenopodium rubrum suspension cells.

The Journal of membrane biology ·Vol. 136 ·No. 1 ·1993-10-00 ·Pages 43-54

Weiser T, Bentrup FW

Abstract

Single channel performance and deactivation currents have been analyzed in the presence of cation channel blockers to reveal pharmacological properties of the slow-activating (SV) cation-selective ion channel in the vacuolar membrane (tonoplast) isolated from suspension cells of Chenopodium rubrum L. At a holding potential of -100 mV, the SV channel showed half-maximal inhibition with 20 mM tetraethylammonium (TEA), 7 microM 9-amino-acridine, 6 microM (+)-tubocurarine, 300 nM quinacrine, and 35 microM quinine, respectively. The SV channel is also blocked by charybdotoxin (20 nM at -80 mV) but not by apamine. 9-Amino-acridine, (+)-tubocurarine and quinacrine act in a voltage-dependent fashion, binding to the open channel and to different sites along the transmembrane voltage profile according to Woodhull (J. Gen. Physiol. 61:687-708, 1973). No binding site could be specified for charybdotoxin, which binds to the closed channel, and for quinine. Except for quinine, all tested blockers were effective only if added to the cytoplasmic side of the tonoplast. A structural relationship between the SV channel and Maxi-K channels in animal systems is inferred.

MeSH Terms
Animals Cations Charybdotoxin Intracellular Membranes/chemistry,drug effects,physiology Ion Channels/analysis,physiology Membrane Potentials/drug effects,physiology Plant Cells Plant Physiological Phenomena Quinacrine/pharmacology Quinine/pharmacology Scorpion Venoms/pharmacology Tetradecanoylphorbol Acetate/pharmacology Tubocurarine/pharmacology
Chemicals
Cations Ion Channels Scorpion Venoms Charybdotoxin Quinine Quinacrine Tetradecanoylphorbol Acetate Tubocurarine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Weiser T
Institut für Allgemeine Botanik und Pfanzenphysiologie, Justus-Liebig-Universität, Giessen, Germany.
Bentrup F W
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF00241488
Published
1993-10-00
Pages
43-54
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00241488 View journal details
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