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PMID: 7504739 Published · ppublish English Journal Article

Apoptosis induced in CD4+ cells expressing gp160 of human immunodeficiency virus type 1.

Journal of virology ·Vol. 68 ·No. 1 ·1994-01-00 ·Pages 390-9

Lu YY, Koga Y, Tanaka K, Sasaki M, Kimura G, Nomoto K

Abstract

In a previous study (Y. Koga, M. Sasaki, H. Yoshida, H. Wigzell, G. Kimura, and K. Nomoto, J. Immunol. 144:94-102, 1990), we demonstrated that the expression of gp160, a precursor form of envelope glycoprotein of human immunodeficiency virus type 1, in CD4+ cells causes the downregulation of surface CD4 and single-cell killing by forming intracellular gp160-CD4 complex. In the present study we investigated the events that lead to cell death in CD4+ cells expressing gp160. We found that apoptosis is induced in cells undergoing single-cell death. Moreover, even the cell clone, which expresses so little gp160 that it does not exhibit any apparent cytopathic effects, such as the inhibition of cell growth, was found to be highly susceptible to the apoptosis induction by the anti-Fas monoclonal antibody.

MeSH Terms
Antigens, Surface/pharmacology Apoptosis/drug effects Base Sequence CD4 Antigens/biosynthesis CD4-Positive T-Lymphocytes/cytology,immunology,ultrastructure Cell Line Cytopathogenic Effect, Viral DNA Damage Down-Regulation Gene Products, env/biosynthesis,pharmacology HIV Envelope Protein gp160 HIV-1/physiology Humans Molecular Sequence Data Protein Precursors/biosynthesis,pharmacology Recombinant Proteins/biosynthesis Transfection fas Receptor
Chemicals
Antigens, Surface CD4 Antigens Gene Products, env HIV Envelope Protein gp160 Protein Precursors Recombinant Proteins fas Receptor
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lu Y Y
Department of Immunology, Kyushu University, Fukuoka, Japan.
Koga Y
Tanaka K
Sasaki M
Kimura G
Nomoto K
References (28)
28 references, click to expand
  1. The cytopathic effect of HIV is associated with apoptosis.
    Virology. 1991 Dec;185(2):829-39 PMID: 1683728
  2. Cytopathic effect determined by the amount of CD4 molecules in human cell lines expressing envelope glycoprotein of HIV.
    J Immunol. 1990 Jan 1;144(1):94-102 PMID: 2404066
  3. Death of mature T cells by separate ligation of CD4 and the T-cell receptor for antigen.
    Nature. 1990 Sep 20;347(6290):286-9 PMID: 2144894
  4. Anti-Fas monoclonal antibody is cytocidal to human immunodeficiency virus-infected cells without augmenting viral replication.
    Proc Natl Acad Sci U S A. 1990 Dec;87(24):9620-4 PMID: 1702216
  5. Apoptosis as a mechanism of cell death in cultured T lymphoblasts acutely infected with HIV-1.
    J Clin Invest. 1991 May;87(5):1710-5 PMID: 2022741
  6. Disturbance of nuclear transport of proteins in CD4+ cells expressing gp160 of human immunodeficiency virus.
    J Virol. 1991 Oct;65(10):5609-12 PMID: 1895408
  7. Simultaneous analysis of immunophenotype and apoptosis of murine thymocytes by single laser flow cytometry.
    Cytometry. 1992;13(8):809-21 PMID: 1458998
  8. HIV infection is active and progressive in lymphoid tissue during the clinically latent stage of disease.
    Nature. 1993 Mar 25;362(6418):355-8 PMID: 8455722
  9. Massive covert infection of helper T lymphocytes and macrophages by HIV during the incubation period of AIDS.
    Nature. 1993 Mar 25;362(6418):359-62 PMID: 8096068
  10. Apoptosis.
    Immunol Today. 1993 Mar;14(3):126-30 PMID: 8466628
  11. Direct and indirect mechanisms mediating apoptosis during HIV infection: contribution to in vivo CD4 T cell depletion.
    Semin Immunol. 1993 Jun;5(3):187-94 PMID: 8102263
  12. Quantitative changes in T helper or T suppressor/cytotoxic lymphocyte subsets that distinguish acquired immune deficiency syndrome from other immune subset disorders.
    Am J Med. 1984 Jan;76(1):95-100 PMID: 6229182
  13. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
    Biochem J. 1956 Feb;62(2):315-23 PMID: 13293190
  14. Chromatin cleavage in apoptosis: association with condensed chromatin morphology and dependence on macromolecular synthesis.
    J Pathol. 1984 Jan;142(1):67-77 PMID: 6422024
  15. Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):7161-5 PMID: 6438633
  16. Molecular characterization of human T-cell leukemia (lymphotropic) virus type III in the acquired immune deficiency syndrome.
    Science. 1984 Dec 7;226(4679):1165-71 PMID: 6095449
  17. Complete nucleotide sequence of the AIDS virus, HTLV-III.
    Nature. 1985 Jan 24-30;313(6000):277-84 PMID: 2578615
  18. Long-term cultures of HTLV-III--infected T cells: a model of cytopathology of T-cell depletion in AIDS.
    Science. 1986 Feb 21;231(4740):850-3 PMID: 2418502
  19. Susceptibility to infection by the human immunodeficiency virus (HIV) correlates with T4 expression in a parental monocytoid cell line and its subclones.
    Virology. 1987 Apr;157(2):359-65 PMID: 3103330
  20. A major mechanism of human immunodeficiency virus-induced cell killing does not involve cell fusion.
    J Virol. 1987 Oct;61(10):3114-9 PMID: 2957508
  21. The human immunodeficiency virus: infectivity and mechanisms of pathogenesis.
    Science. 1988 Feb 5;239(4840):617-22 PMID: 3277274
  22. Envelope glycoprotein of HIV induces interference and cytolysis resistance in CD4+ cells: mechanism for persistence in AIDS.
    Cell. 1988 May 6;53(3):483-96 PMID: 2966682
  23. Characterization of a human immunodeficiency virus neutralizing monoclonal antibody and mapping of the neutralizing epitope.
    J Virol. 1988 Jun;62(6):2107-14 PMID: 2452899
  24. Tumor necrosis factor alpha and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor kappa B.
    Proc Natl Acad Sci U S A. 1989 Apr;86(7):2336-40 PMID: 2494664
  25. Tumor necrosis factor alpha induces expression of human immunodeficiency virus in a chronically infected T-cell clone.
    Proc Natl Acad Sci U S A. 1989 Apr;86(7):2365-8 PMID: 2784570
  26. A cell-killing monoclonal antibody (anti-Fas) to a cell surface antigen co-downregulated with the receptor of tumor necrosis factor.
    J Exp Med. 1989 May 1;169(5):1747-56 PMID: 2469768
  27. Depletion of the surface CD4 molecule by the envelope protein of human immunodeficiency virus expressed in a human CD4+ monocytoid cell line.
    J Virol. 1989 Sep;63(9):3748-54 PMID: 2788223
  28. Intracellular distribution of the envelope glycoprotein of human immunodeficiency virus and its role in the production of cytopathic effect in CD4+ and CD4- human cell lines.
    J Virol. 1990 Oct;64(10):4661-71 PMID: 2204721
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.68.1.390-399.1994
Published
1994-01-00
Pages
390-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC236299
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.68.1.390-399.1994 PMC Full Text View journal details
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