Home LiteratureArticle Details
PMID: 7504264 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human immunodeficiency virus infection of human brain capillary endothelial cells occurs via a CD4/galactosylceramide-independent mechanism.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 22 ·1993-11-15 ·Pages 10474-8

Moses AV, Bloom FE, Pauza CD, Nelson JA

Abstract

Neuropathologic studies of AIDS patients have shown that brain capillary endothelial cells are a cellular target for human immunodeficiency virus (HIV) in vivo. We have established in vitro cultures of primary human brain capillary endothelial (HBCE) cells. Using this model system, we have shown a significant HIV infection of HBCE cells that is productive yet noncytopathic. The infection is mediated by a cellular interaction with gp120 that does not involve CD4 or galactosylceramide. HIV infection of HBCE cells may contribute to AIDS-associated neuropathology by disturbing the physiology of the endothelium and directly or indirectly facilitating dissemination of virus to the central nervous system.

MeSH Terms
CD4 Antigens/metabolism Cells, Cultured Endothelium, Vascular/microbiology Epitopes Fluorescent Antibody Technique Galactosylceramides/metabolism HIV Envelope Protein gp120/immunology,metabolism HIV Infections/metabolism HIV-1/growth & development Humans In Vitro Techniques Receptors, Virus/metabolism Virus Replication
Chemicals
CD4 Antigens Epitopes Galactosylceramides HIV Envelope Protein gp120 Receptors, Virus
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Moses A V
Department of Microbiology and Immunology, Oregon Health Sciences University, Portland 97201.
Bloom F E
Pauza C D
Nelson J A
References (46)
46 references, click to expand
  1. Human immunodeficiency virus in brain biopsies of patients with AIDS and progressive encephalopathy.
    J Infect Dis. 1987 May;155(5):870-6 PMID: 3644852
  2. AIDS subacute encephalitis. Identification of HIV-infected cells.
    Am J Pathol. 1987 Mar;126(3):403-10 PMID: 3548405
  3. Human immunodeficiency virus can productively infect cultured human glial cells.
    Proc Natl Acad Sci U S A. 1987 May;84(10):3526-30 PMID: 3472222
  4. Immunohistochemical localization of human and simian immunodeficiency viral antigens in fixed tissue sections.
    Am J Pathol. 1987 May;127(2):199-205 PMID: 3472469
  5. HIV antigen in the brains of patients with the AIDS dementia complex.
    Ann Neurol. 1987 May;21(5):490-6 PMID: 3296948
  6. The brain in AIDS: central nervous system HIV-1 infection and AIDS dementia complex.
    Science. 1988 Feb 5;239(4840):586-92 PMID: 3277272
  7. Characterization of a human immunodeficiency virus neutralizing monoclonal antibody and mapping of the neutralizing epitope.
    J Virol. 1988 Jun;62(6):2107-14 PMID: 2452899
  8. Neuronal cell killing by the envelope protein of HIV and its prevention by vasoactive intestinal peptide.
    Nature. 1988 Oct 13;335(6191):639-42 PMID: 2845276
  9. Soluble CD4 blocks the infectivity of diverse strains of HIV and SIV for T cells and monocytes but not for brain and muscle cells.
    Nature. 1989 Jan 26;337(6205):368-70 PMID: 2536142
  10. Vascular endothelium in immunology and infectious disease.
    Rev Infect Dis. 1989 Mar-Apr;11(2):273-83 PMID: 2495565
  11. Treatment of HIV tissue culture infection with monoclonal antibody-ricin A chain conjugates.
    J Immunol. 1989 May 1;142(9):3070-5 PMID: 2540236
  12. CD4-independent infection of human neural cells by human immunodeficiency virus type 1.
    J Virol. 1989 Jun;63(6):2527-33 PMID: 2786088
  13. Infection of brain cells by diverse human immunodeficiency virus isolates: role of CD4 as receptor.
    J Gen Virol. 1989 Oct;70 ( Pt 10):2653-60 PMID: 2677235
  14. CD4-independent, productive infection of a neuronal cell line by human immunodeficiency virus type 1.
    J Virol. 1990 Mar;64(3):1383-7 PMID: 2304148
  15. HIV-1 coat protein neurotoxicity prevented by calcium channel antagonists.
    Science. 1990 Apr 20;248(4953):364-7 PMID: 2326646
  16. Tat protein of HIV-1 stimulates growth of cells derived from Kaposi's sarcoma lesions of AIDS patients.
    Nature. 1990 May 3;345(6270):84-6 PMID: 2184372
  17. Cultured human brain capillary endothelial cells are permissive for infection by human cytomegalovirus.
    Virology. 1990 May;176(1):266-73 PMID: 2158692
  18. Specific tropism of HIV-1 for microglial cells in primary human brain cultures.
    Science. 1990 Aug 3;249(4968):549-53 PMID: 2200125
  19. Infection of brain microglial cells by human immunodeficiency virus type 1 is CD4 dependent.
    J Virol. 1991 Feb;65(2):736-42 PMID: 1702842
  20. Evidence for neurotoxic activity of tat from human immunodeficiency virus type 1.
    J Virol. 1991 Feb;65(2):961-7 PMID: 1898974
  21. Secretion of neurotoxins by mononuclear phagocytes infected with HIV-1.
    Science. 1990 Dec 14;250(4987):1593-6 PMID: 2148832
  22. Human immunodeficiency virus-infected macrophages produce soluble factors that cause histological and neurochemical alterations in cultured human brains.
    J Clin Invest. 1991 Feb;87(2):503-12 PMID: 1671392
  23. An IgG human monoclonal antibody that reacts with HIV-1/GP120, inhibits virus binding to cells, and neutralizes infection.
    J Immunol. 1991 Jun 15;146(12):4325-32 PMID: 1710248
  24. Use of a focal infectivity assay for testing susceptibility of HIV to antiviral agents.
    Biotechniques. 1991 Mar;10(3):336-42 PMID: 2064772
  25. HIV-1, macrophages, glial cells, and cytokines in AIDS nervous system disease.
    FASEB J. 1991 Jul;5(10):2391-7 PMID: 2065887
  26. Inhibition of entry of HIV-1 in neural cell lines by antibodies against galactosyl ceramide.
    Science. 1991 Jul 19;253(5017):320-3 PMID: 1857969
  27. Localization of simian immunodeficiency virus in the central nervous system of rhesus monkeys.
    Am J Pathol. 1991 Sep;139(3):609-21 PMID: 1716047
  28. Primary cultures of endothelial cells from the human liver sinusoid are permissive for human immunodeficiency virus type 1.
    Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1582-6 PMID: 1371878
  29. Human immunodeficiency virus infection in microglia: correlation between cells infected in the brain and cells cultured from infectious brain tissue.
    Ann Neurol. 1992 Apr;31(4):361-5 PMID: 1586136
  30. Cytokine regulation of endothelial cell function.
    FASEB J. 1992 May;6(8):2591-9 PMID: 1592209
  31. Human immunodeficiency virus type-1 (HIV-1) infection of endothelial cells in vitro: a virological, ultrastructural and immuno-cytochemical approach.
    J Submicrosc Cytol Pathol. 1992 Apr;24(2):155-61 PMID: 1376212
  32. Recent advances in understanding brain capillary function.
    Ann Neurol. 1983 Oct;14(4):389-95 PMID: 6139057
  33. HTLV-III infection in brains of children and adults with AIDS encephalopathy.
    Science. 1985 Jan 11;227(4683):177-82 PMID: 2981429
  34. Isolation of AIDS-associated retroviruses from cerebrospinal fluid and brain of patients with neurological symptoms.
    Lancet. 1985 Sep 14;2(8455):586-8 PMID: 2863599
  35. Isolation of HTLV-III from cerebrospinal fluid and neural tissues of patients with neurologic syndromes related to the acquired immunodeficiency syndrome.
    N Engl J Med. 1985 Dec 12;313(24):1493-7 PMID: 2999591
  36. HIV infects glomerular endothelial and mesangial but not epithelial cells in vitro.
    Kidney Int. 1992 Apr;41(4):956-60 PMID: 1513116
  37. Interaction of human immunodeficiency virus with human macrovascular endothelial cells in vitro.
    AIDS Res Hum Retroviruses. 1992 Sep;8(9):1567-70 PMID: 1457202
  38. Pathogenesis and therapy of HIV-1 infection of the central nervous system.
    AIDS. 1992 Dec;6(12):1411-26 PMID: 1337255
  39. HTLV-III/LAV-like retrovirus particles in the brains of patients with AIDS encephalopathy.
    AIDS Res. 1984-1985;1(6):447-54 PMID: 6100783
  40. Detection of AIDS virus in macrophages in brain tissue from AIDS patients with encephalopathy.
    Science. 1986 Sep 5;233(4768):1089-93 PMID: 3016903
  41. Cellular localization of human immunodeficiency virus infection within the brains of acquired immune deficiency syndrome patients.
    Proc Natl Acad Sci U S A. 1986 Sep;83(18):7089-93 PMID: 3018755
  42. Immunohistochemical identification of HTLV-III antigen in brains of patients with AIDS.
    Ann Neurol. 1986 Sep;20(3):289-95 PMID: 3532930
  43. Human T-cell lymphotropic virus type III infection of the central nervous system. A preliminary in situ analysis.
    JAMA. 1986 Nov 7;256(17):2360-4 PMID: 3639953
  44. Plasmalemmal proteins of cultured vascular endothelial cells exhibit apical-basal polarity: analysis by surface-selective iodination.
    J Cell Biol. 1986 Dec;103(6 Pt 1):2389-402 PMID: 3782302
  45. Shedding and interspecies type sero-reactivity of the envelope glycopolypeptide gp120 of the human immunodeficiency virus.
    J Gen Virol. 1986 Nov;67 ( Pt 11):2533-8 PMID: 2431105
  46. Neurologic abnormalities and recovery of human immunodeficiency virus from cerebrospinal fluid.
    Ann Intern Med. 1987 May;106(5):692-5 PMID: 3646001
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.22.10474
Published
1993-11-15
Pages
10474-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC47799
Subset
IM
Grants
NIAID NIH HHS · AI 24178 · United States
NIAID NIH HHS · AI 24591 · United States
NIMH NIH HHS · NIMH 47680 · United States
External Links
PubMed source DOI: 10.1073/pnas.90.22.10474 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2