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PMID: 7500052 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

gro-beta, a -C-X-C- chemokine, is an angiogenesis inhibitor that suppresses the growth of Lewis lung carcinoma in mice.

The Journal of experimental medicine ·Vol. 182 ·No. 6 ·1995-12-01 ·Pages 2069-77

Cao Y, Chen C, Weatherbee JA, Tsang M, Folkman J

Abstract

We have found that two chemokines, recombinant gro-alpha and gro-beta, specifically inhibit growth factor-stimulated proliferation of capillary endothelial cells in a dose-dependent manner, whereas gro-gamma has no inhibitory effect. In vivo, gro-beta inhibits blood vessel formation in the chicken chorioallantoic membrane assay. It is sufficiently potent to effectively suppress basic fibroblast growth factor-induced corneal neovascularization after systemic administration in mice. Further, gro-beta significantly inhibits the growth of murine Lewis lung carcinoma in syngeneic C57B16/J and immunodeficient nude mice without toxicity. In vitro, Lewis lung carcinoma cells are completely insensitive to recombinant gro-beta at high concentrations that significantly inhibit endothelial cell proliferation. This finding supports the conclusion that gro-beta inhibits Lewis lung tumor growth by suppression of tumor-induced neovascularization.

MeSH Terms
Amino Acid Sequence Animals Carcinoma, Lewis Lung/drug therapy Chemokine CXCL1 Chemokines/chemistry,pharmacology Chemokines, CXC Chemotactic Factors/pharmacology Chick Embryo Consensus Sequence Growth Inhibitors/pharmacology Growth Substances/pharmacology Humans Intercellular Signaling Peptides and Proteins Mice Mice, Inbred C57BL Mice, Nude Molecular Sequence Data Neovascularization, Pathologic/prevention & control Recombinant Proteins Sequence Alignment Sequence Homology, Amino Acid
Chemicals
CXCL1 protein, human Chemokine CXCL1 Chemokines Chemokines, CXC Chemotactic Factors Cxcl1 protein, mouse Growth Inhibitors Growth Substances Intercellular Signaling Peptides and Proteins Recombinant Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cao Y
Department of Surgery, Harvard Medical School, Boston, Massachusetts 02115, USA.
Chen C
Weatherbee J A
Tsang M
Folkman J
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.182.6.2069
Published
1995-12-01
Pages
2069-77
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2192268
Subset
IM
Grants
NCI NIH HHS · P01-CA-45548 · United States
External Links
PubMed source DOI: 10.1084/jem.182.6.2069 PMC Full Text View journal details
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