Cloning and primary structure of a new hemolysin gene from Bacillus cereus.

Biochimica et biophysica acta ·Vol. 1264 ·No. 2 ·1995-11-07 ·Pages 151-4

Baida GE, Kuzmin NP

Abstract

A new hemolysin gene from Bacillus cereus VKM-B164 was cloned in Escherichia coli and sequenced. Deduced protein consists of 219 amino acids and has a molecular mass of 24.4 kDa. It has been concluded that the hemolysin, named 'hemolysin III', is distinct from the B. cereus hemolysins reported previously: cereolysin, sphingomyelinase, cereolysin AB, hemolysin II, and 'cereolysin-like' hemolysin (Honda, T., Shiba, A., Seo, S., Yamamoto, J., Matsuyama, J. and Miwatani, T. (1991) FEMS Microbiol. Lett. 79, 205-210).

MeSH Terms

Amino Acid Sequence Bacillus cereus/genetics Base Sequence Blotting, Southern Cloning, Molecular/methods DNA, Bacterial/chemistry,metabolism Escherichia coli Genes, Bacterial Hemolysin Proteins/biosynthesis,chemistry,genetics Molecular Sequence Data Recombinant Proteins/biosynthesis,chemistry Restriction Mapping

Chemicals

DNA, Bacterial Hemolysin Proteins Recombinant Proteins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Baida G E

Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.

Kuzmin N P

Article Info

Journal

Biochimica et biophysica acta

Abbr.

Biochim Biophys Acta

ISSN

0006-3002

DOI

10.1016/0167-4781(95)00150-f

Published

1995-11-07

Pages

151-4

Language

English

Region

Netherlands

NLM ID

0217513

Subset

Databases

GENBANK

X84058

External Links

PubMed source DOI: 10.1016/0167-4781(95)00150-f

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Cloning and primary structure of a new hemolysin gene from Bacillus cereus.

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