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PMID: 7489705 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of band 3 anion exchanger induces chloride current and taurine transport: structure-function analysis.

The EMBO journal ·Vol. 14 ·No. 21 ·1995-11-01 ·Pages 5158-69

Fiévet B, Gabillat N, Borgese F, Motais R

Abstract

Most, but not all, cell types release intracellular organic solutes (e.g. taurine) in response to cell swelling to achieve cell volume regulation. Although this efflux is blocked by classical inhibitors of the electroneutral anion exchanger band 3 (AE1), it is thought to involve an anion channel. The role of band 3 in volume-dependent taurine transport was determined by expressing, in Xenopus oocytes, band 3 from erythrocytes which do (trout) or do not (mouse) release taurine when swollen. AE1 of both species elicited anion exchange activity, but only trout band 3 showed chloride channel activity and taurine transport. Chimeras constructed from trout and mouse band 3 allowed the identification of some protein domains critically associated with channel activity and taurine transport. The data provide evidence that swelling-induced taurine movements occur via an anion channel which is dependent on, or controlled by, band 3. They suggest the involvement of proteins of the band 3 (AE) family in cell volume regulation.

MeSH Terms
Amino Acid Sequence Animals Anion Exchange Protein 1, Erythrocyte/chemistry,genetics,metabolism Base Sequence Biological Transport, Active Chlorides/metabolism Gene Deletion Gene Transfer Techniques Ion Channels/metabolism Mice Molecular Sequence Data Recombinant Fusion Proteins/chemistry,genetics,metabolism Taurine/metabolism Trout Xenopus
Chemicals
Anion Exchange Protein 1, Erythrocyte Chlorides Ion Channels Recombinant Fusion Proteins Taurine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fiévet B
Département de Biologie, Cellulaire et Moléculaire, CEA, Laboratoire Jean Maetz, Villefranche-sur-mer, France.
Gabillat N
Borgese F
Motais R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-11-01
Pages
5158-69
Language
English
Region
England
NLM ID
8208664
PMCID
PMC394623
Subset
IM
Databases
GENBANK
Z50848
External Links
PubMed source PMC Full Text View journal details
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