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PMID: 7479941 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification and characterization of an immunophilin expressed during the clonal expansion phase of adipocyte differentiation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 24 ·1995-11-21 ·Pages 11081-5

Yeh WC, Li TK, Bierer BE, McKnight SL

Abstract

Mouse 3T3-L1 cells differentiate into fat-laden adipocytes in response to a cocktail of adipogenic hormones. This conversion process occurs in two discrete steps. During an early clonal expansion phase, confluent 3T3-L1 cells proliferate and express the products of the beta and delta members of the CCAAT/enhancer binding protein (C/EBP) family of transcription factors. The cells subsequently arrest mitotic growth, induce the expression of the alpha form of C/EBP, and acquire the morphology of fully differentiated adipocytes. Many of the genes induced during the terminal phase of adipocyte conversion are directly activated by C/EBP alpha, and gratuitous expression of this transcription factor is capable of catalyzing adipose conversion in a number of different cultured cell lines. The genetic program undertaken during the clonal expansion phase of 3T3-L1 differentiation, controlled in part by C/EBP beta and C/EBP delta, is less clearly understood. To study the molecular events occurring during clonal expansion, we have identified mRNAs that selectively accumulate during this phase of adipocyte conversion. One such mRNA encodes an immunophilin hereby designated FKBP51. In this report we provide the initial molecular characterization of FKBP51.

MeSH Terms
Adipocytes/cytology,enzymology Amino Acid Isomerases/genetics Amino Acid Sequence Animals Carrier Proteins/genetics,metabolism Cell Differentiation Clone Cells DNA-Binding Proteins/genetics,metabolism Gene Expression Heat-Shock Proteins/genetics,metabolism Mice Molecular Sequence Data Peptidylprolyl Isomerase Polyenes/metabolism RNA, Messenger/genetics Sequence Alignment Sequence Homology, Amino Acid Sirolimus Tacrolimus/metabolism Tacrolimus Binding Proteins Tissue Distribution
Chemicals
Carrier Proteins DNA-Binding Proteins Heat-Shock Proteins Polyenes RNA, Messenger Amino Acid Isomerases Tacrolimus Binding Proteins Peptidylprolyl Isomerase Sirolimus Tacrolimus
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yeh W C
Johns Hopkins University, Baltimore, MD 21218, USA.
Li T K
Bierer B E
McKnight S L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.24.11081
Published
1995-11-21
Pages
11081-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC40575
Subset
IM
Grants
NIAID NIH HHS · AI 32514 · United States
Databases
GENBANK
U36220
External Links
PubMed source DOI: 10.1073/pnas.92.24.11081 PMC Full Text View journal details
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