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PMID: 7479064 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The purine-rich trinucleotide repeat sequences d(CAG)15 and d(GAC)15 form hairpins.

Nucleic acids research ·Vol. 23 ·No. 20 ·1995-10-25 ·Pages 4055-7

Yu A, Dill J, Mitas M

Abstract

The structures of single-stranded (ss) oligonucleotides containing (CAG)15 [ss(CAG)15] or (GAC)15 [ss(GAC)15] were examined. At 10 degrees C, the electrophoretic mobilites of the two DNAs were similar to ss(CTG)15, a DNA that forms a hairpin containing base paired and/or stacked thymines. At 37 degrees C in 50 mM NaCl, single-strand-specific P1 nuclease cleaved the G33-G36 phosphodiesters of ss(GAC)15, and the G32-A34, G35-C36 phosphodiesters of ss(CAG)15 (where the loop apex of both DNAs = A34). Electrophoretic mobility melting profiles indicated that the melting temperature (Tm) of ss(CAG)15 in low (approximately 1 mM Na+) ionic strength was 38 degrees C. In contrast, the Tm of ss(GAC)15 was 49 degrees C, a value similar to the Tm of ss(CTG)15. These results provide evidence that ss(GAC)15 and ss(CAG)15 form similar, but distinguishable hairpin structures.

MeSH Terms

Base Sequence DNA/chemistry Deoxyadenosines Deoxyguanosine Molecular Sequence Data Nucleic Acid Conformation Nucleic Acid Denaturation Oligodeoxyribonucleotides/chemical synthesis,chemistry Single-Strand Specific DNA and RNA Endonucleases Temperature Trinucleotide Repeats

Chemicals

Deoxyadenosines Oligodeoxyribonucleotides DNA Single-Strand Specific DNA and RNA Endonucleases Deoxyguanosine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Yu A

Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater 74078, USA.

Dill J

Mitas M

References (8)

8 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/23.20.4055

Published

1995-10-25

Pages

4055-7

Language

English

Region

England

NLM ID

0411011

PMCID

PMC307342

Subset

External Links

PubMed source DOI: 10.1093/nar/23.20.4055 PMC Full Text View journal details

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The purine-rich trinucleotide repeat sequences d(CAG)15 and d(GAC)15 form hairpins.

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