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PMID: 7479058 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Aberrant pre-mRNA maturation is caused by LINE insertions into introns of the white gene of Drosophila melanogaster.

Nucleic acids research ·Vol. 23 ·No. 20 ·1995-10-25 ·Pages 4015-22

Lajoinie O, Drake ME, Dastugue B, Vaury C

Abstract

Insertional mutagenesis screens have provided thousands of mutant alleles for analysing genes of varied functions in Drosophila melanogaster. We here document mechanisms of insertional mutagenesis by a LINE element, the I factor, by determining the molecular structure of RNAs produced from two alleles of the white gene of D.melanogaster, wIR1 and wIR6. These alleles result from insertion of the I factor into introns of the gene. We show that sequences present within the element direct aberrant splicing and termination events. When the I factor is inserted within the white first intron it may lead to the use of a cryptic 3' splice site which does not contain the dinucleotide AG. This splicing gives rise to a chimeric messenger RNA whose synthesis is controlled differently in tissues where the mutated gene is expressed. When the I factor is inserted within the white last intron it induces synthesis of truncated mRNAs. These results provide, for the first time, mechanisms for I factor insertional mutagenesis. They are discussed in the more general context of RNA processing in Drosophila and the evolution of eukaryotic gene introns.

MeSH Terms
ATP-Binding Cassette Transporters Alleles Amino Acid Sequence Animals Base Sequence DNA Transposable Elements Drosophila Proteins Drosophila melanogaster/genetics Eye Proteins Gene Expression Regulation, Developmental Genes, Insect/genetics Insect Hormones/genetics Introns/genetics Molecular Sequence Data Mutagenesis, Insertional/genetics Organ Specificity RNA Precursors/genetics RNA Splicing/genetics RNA, Messenger/analysis
Chemicals
ATP-Binding Cassette Transporters DNA Transposable Elements Drosophila Proteins Eye Proteins Insect Hormones RNA Precursors RNA, Messenger w protein, Drosophila
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lajoinie O
INSERM U384, Faculté de Médecine, Clermont-Ferrand, France.
Drake M E
Dastugue B
Vaury C
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/23.20.4015
Published
1995-10-25
Pages
4015-22
Language
English
Region
England
NLM ID
0411011
PMCID
PMC307336
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/23.20.4015 PMC Full Text View journal details
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