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PMID: 7463558 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nucleic acids of respiratory syncytial virus.

Journal of virology ·Vol. 36 ·No. 3 ·1980-12-00 ·Pages 837-46

Lambert DM, Pons MW, Mbuy GN, Dorsch-Hasler K

Abstract

Analysis of purified respiratory syncytial virus revealed that the virion RNA was composed of 50S, 28S, 18S, and 4S species. The 18S and 28S species were presumed to represent host rRNA since virus grown in actinomycin D-treated cells contained only 50S and 4S RNAs. Actinomycin D treatment stimulated production of infectious respiratory syncytial virus 5- to 10-fold. The 50S virion RNA was shown to hybridize with polyadenylated mRNA's isolated from infected cells, indicating that respiratory syncytial virus RNA is of negative-strand sense. Six mRNA's were identified by polyacrylamide gel electrophoresis.

MeSH Terms
Cell Line Dactinomycin/pharmacology Electrophoresis, Polyacrylamide Gel Molecular Weight RNA, Messenger/analysis RNA, Viral/analysis,biosynthesis Respiratory Syncytial Viruses/analysis
Chemicals
RNA, Messenger RNA, Viral Dactinomycin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lambert D M
Pons M W
Mbuy G N
Dorsch-Hasler K
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24 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.36.3.837-846.1980
Published
1980-12-00
Pages
837-46
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353711
Subset
IM
Grants
NIAID NIH HHS · AI 14133 · United States
External Links
PubMed source DOI: 10.1128/JVI.36.3.837-846.1980 PMC Full Text View journal details
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