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PMID: 7460390 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Immune status in Crohn's disease. V. Decreased in vitro natural killer cell activity in peripheral blood.

Clinical and experimental immunology ·Vol. 42 ·No. 1 ·1980-10-00 ·Pages 41-9

Auer IO, Ziemer E, Sommer H

Abstract

To identify and measure the spontaneous cell-mediated cytotoxicity of natural killer cells, we used the lysis of an established lymphoblastoid cell line as target in a 4-hr 51Cr-release assay. Mononuclear cell suspensions of the peripheral blood of thirty-four patients with Crohn's disease (group CD), eleven patients with inflammatory bowel disease other than CD or ulcerative colitis (group D) and forty-five healthy subjects matched for both age and sex with the patients were studied. Depletion of phagocytic, plastic-adherent cells ('purified suspensions') led to a significant increase of the natural killer cell activity as compared with unseparated suspensions. This was seen to occur in all groups. In CD patients the natural killer cell activity was significantly below normal levels in both unseparated and 'purified' suspensions. This was independent of disease duration. In 'purified' suspensions the natural killer cell activity was inversely related to the disease activity. In group D the natural cytotoxicity was significantly lower in unseparated suspensions than that in healthy controls. In 'purified' suspensions it was still slightly lower than in healthy controls.

MeSH Terms
Adult Antibody-Dependent Cell Cytotoxicity Crohn Disease/immunology Cytotoxicity, Immunologic Female Humans Killer Cells, Natural/immunology Leukocyte Count Male Middle Aged
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Auer I O
Ziemer E
Sommer H
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30 references, click to expand
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Article Info
Journal
Clinical and experimental immunology
Abbr.
Clin Exp Immunol
ISSN
0009-9104
Published
1980-10-00
Pages
41-9
Language
English
Region
England
NLM ID
0057202
PMCID
PMC1537072
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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