A rapid method for the preparation of relatively pure metabolically competent synaptosomes from rat brain.

The Biochemical journal ·Vol. 176 ·No. 2 ·1978-11-15 ·Pages 365-70

Booth RF, Clark JB

Abstract

A rapid (less than 2h) method is described for the preparation of synaptosomes from rat brain by using a discontinuous Ficoll/sucrose gradient by a flotation technique. These synaptosomes are metabolically active and minimally (less than 5%) contaminated with 'free' mitochondria as judged by marker-enzyme assays and electron microscopy.

MeSH Terms

Animals Brain/metabolism,ultrastructure Cell Fractionation/methods Centrifugation, Density Gradient/methods In Vitro Techniques Male Methods Mitochondria Oxygen Consumption Rats Synaptosomes/metabolism,ultrastructure

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Booth R F

Clark J B

References (20)

20 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1760365

Published

1978-11-15

Pages

365-70

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1186243

Subset

External Links

PubMed source DOI: 10.1042/bj1760365 PMC Full Text View journal details

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A rapid method for the preparation of relatively pure metabolically competent synaptosomes from rat brain.

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