Response to chemotherapy of EMT6 spheroids as measured by growth delay and cell survival.

British journal of cancer ·Vol. 42 ·No. 2 ·1980-08-00 ·Pages 297-304

Twentyman PR

Abstract

Multicellular tumour spheroids of the EMT6 mouse tumour line have been grown in a static (i.e. non-spinner) culture system to a mean spheroid diameter of 250 mum. Samples of spheroids were then exposed for 1 h to graded concentrations of various cytotoxic drugs, and the response assayed by both growth delay and survival of clonogenic cells. For nitrogen mustard (HN2), melphalan, BCNU, CCNU and cis-platinum, a considerable recovery in measured cell survival was seen if correlation between growth delay and cell survival (measured at 24 h) was observed for these 5 agents. For adriamycin, actinomycin D and 5-fluorouracil, no increase in measured cell survival was seen for a 24h delay in assay, and these agents produced longer growth delays for a given level of cell kill.

MeSH Terms

Animals Antineoplastic Agents/pharmacology Cell Division/drug effects Cell Survival/drug effects Cells, Cultured Clone Cells/drug effects Dose-Response Relationship, Drug Mammary Neoplasms, Experimental/drug therapy Mice Models, Biological Time Factors

Chemicals

Antineoplastic Agents

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Twentyman P R

References (14)

14 references, click to expand

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Article Info

Journal

British journal of cancer

Abbr.

Br J Cancer

ISSN

0007-0920

DOI

10.1038/bjc.1980.230

Published

1980-08-00

Pages

297-304

Language

English

Region

England

NLM ID

0370635

PMCID

PMC2010392

Subset

External Links

PubMed source DOI: 10.1038/bjc.1980.230 PMC Full Text View journal details

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Response to chemotherapy of EMT6 spheroids as measured by growth delay and cell survival.

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