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PMID: 7400308 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Electron microsocpy of plasmic fragments of human fibrinogen as related to trinodular structure of the intact molecule.

The Journal of clinical investigation ·Vol. 66 ·No. 1 ·1980-07-00 ·Pages 50-6

Fowler WE, Fretto LJ, Erickson HP, McKee PA

Abstract

We have examined rotary shadowed, purified plasmic fragments of human fibrinogen with the electron microscope and have determined the relation of these fragments to the intact fibrinogen molecule. Both intact fibrinogen and its earliest cleavage product, fragment X, are trinodular. The next largest product, fragment Y, consists of two linked nodules. The two terminal products, fragments D and E, are single nodules. From measurements of simultaneously shadowed specimens of these different species, we conclude that the outer nodules of the trinodular fibrinogen molecule are the fragment D-containing regions and the central nodule is the fragment E-containing region.

MeSH Terms
Electrophoresis, Polyacrylamide Gel Fibrinogen/analysis Humans Microscopy, Electron Optical Rotatory Dispersion/methods Sodium Dodecyl Sulfate Structure-Activity Relationship
Chemicals
Sodium Dodecyl Sulfate Fibrinogen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fowler W E
Fretto L J
Erickson H P
McKee P A
References (21)
21 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI109834
Published
1980-07-00
Pages
50-6
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC371504
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI109834 PMC Full Text View journal details
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