A low-molecular-weight inhibitor of the neutral proteinase from rat intestinal smooth muscle.

The Biochemical journal ·Vol. 185 ·No. 2 ·1980-02-01 ·Pages 423-33

Carney IT, Curtis CG, Kay JK, Birket N

Abstract

1. Rat intestinal smooth muscle was shown to contain endogenous inhibitory activity towards the neutral trypsin-like muscle proteinase described previously [Beynon & Kay (1978) Biochem. J. 173, 291--298]. 2. Comtamination of the muscle tissue by mucosal, blood and pancreatic inhibitors was shown to be unlikely. 3. The inhibitory activity was resolved into high- and low-molecular-weight components. 4. The low-molecular-weight component was purified to homogeneity. It has a molecular weight of approx. 9000 and was stable over the pH range 3--11. 5. It inhibited the muscle proteinase competitively (Ki congruent to t microM), but had no effect on any of the other proteinases tested. 6. Leupeptin also inhibited the muscle proteinase competitively (Ki congruent to 0.3 microM), whereas the low-molecular weight proteins gastrin, glucagon and insulin B-chain had very little effect. 7. A role for a weakly binding inhibitor in modulating the influence of the neutral proteinase on intracellular protein degradation is considered.

MeSH Terms

Amino Acids/analysis Animals Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel Hydrolysis Intestine, Small Kinetics Leupeptins/pharmacology Malate Dehydrogenase/antagonists & inhibitors Male Molecular Weight Muscle, Smooth/analysis Peptides/pharmacology Protease Inhibitors/isolation & purification,pharmacology Proteins/pharmacology Rats

Chemicals

Amino Acids Leupeptins Peptides Protease Inhibitors Proteins Malate Dehydrogenase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Carney I T

Curtis C G

Kay J K

Birket N

References (25)

25 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1850423

Published

1980-02-01

Pages

423-33

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1161369

Subset

External Links

PubMed source DOI: 10.1042/bj1850423 PMC Full Text View journal details

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A low-molecular-weight inhibitor of the neutral proteinase from rat intestinal smooth muscle.

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