Home LiteratureArticle Details
PMID: 7340813 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The hydrophobicities of cholera toxin, tetanus toxin and their components.

The Biochemical journal ·Vol. 199 ·No. 2 ·1981-11-01 ·Pages 457-60

Ward WH, Britton P, van Heyningen S

Abstract

1. Charge-shift electrophoresis showed that cholera toxin and its subunits have no hydrophobic surfaces. 2. Amino-acid composition and sequence data suggested that the proteins have no masked hydrophobic regions. 3. The A subunit of cholera toxin may interact with polar molecules in the membrane to exert its effect inside the cell. 4. The only hydrophobic part of tetanus toxin was the H-chain.

MeSH Terms
Amino Acids/analysis Chemical Phenomena Chemistry Cholera Toxin/metabolism Electrophoresis, Agar Gel Proteins/metabolism Tetanus Toxin/metabolism Water/metabolism
Chemicals
Amino Acids Proteins Tetanus Toxin Water Cholera Toxin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ward W H
Britton P
van Heyningen S
References (19)
19 references, click to expand
  1. Membrane proteins: amino acid sequence and membrane penetration.
    J Mol Biol. 1974 Aug 25;87(4):853-8 PMID: 4427380
  2. Cholera toxin: interaction of subunits with ganglioside GM1.
    Science. 1974 Feb 15;183(4125):656-7 PMID: 4810267
  3. Characterization of Torpedo californica acetylcholine receptor: its subunit composition and ligand-binding properties.
    Cold Spring Harb Symp Quant Biol. 1976;40:193-202 PMID: 1065525
  4. Binding of ganglioside by the chains of tetanus toxin.
    FEBS Lett. 1976 Sep 15;68(1):5-7 PMID: 964380
  5. Hydrolysis of nicotinamide adenine dinucleotide by choleragen and its A protomer: possible role in the activation of adenylate cyclase.
    Proc Natl Acad Sci U S A. 1976 Dec;73(12):4424-7 PMID: 188038
  6. Charge shift electrophoresis: simple method for distinguishing between amphiphilic and hydrophilic proteins in detergent solution.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):529-32 PMID: 265517
  7. Activation of adenylate cyclase by choleragen.
    Annu Rev Biochem. 1979;48:581-600 PMID: 224804
  8. Interaction of diphtheria toxin fragments A, B and protein crm 45 with liposomes.
    Eur J Biochem. 1979 Oct 15;100(2):483-9 PMID: 510294
  9. Tetanus toxin.
    Pharmacol Ther. 1980;11(1):141-57 PMID: 7001499
  10. ADP-ribosylation of rat liver membrane protein catalysed by heat-labile enterotoxin from E. coli.
    Biochem Biophys Res Commun. 1980 Oct 16;96(3):1024-31 PMID: 7002157
  11. Activation of choleragen by thiol: protein disulfide oxidoreductase.
    J Biol Chem. 1980 Dec 10;255(23):11085-7 PMID: 7440530
  12. The chemistry and biology of cholera toxin.
    CRC Crit Rev Biochem. 1980;9(3):171-206 PMID: 6256124
  13. Photolabelling of cholera toxin subunits during membrane penetration.
    Nature. 1981 Jan 22;289(5795):319-21 PMID: 6256663
  14. Inhibitors of protein synthesis block action of cholera toxin.
    Biochem Biophys Res Commun. 1981 Feb 12;98(3):677-84 PMID: 6261748
  15. Sequence homologies between A subunits of Escherichia coli and Vibrio cholerae enterotoxins.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):50-4 PMID: 7017718
  16. Is the receptor-mediated endocytosis of cholera toxin A pre-requisite for its activation of adenylate cyclase in intact rat hepatocytes?
    FEBS Lett. 1981 Jun 15;128(2):289-92 PMID: 7262320
  17. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.
    Biochem J. 1963 Oct;89:114-23 PMID: 14097352
  18. On the average hydrophobicity of proteins and the relation between it and protein structure.
    J Theor Biol. 1967 Aug;16(2):187-211 PMID: 6048539
  19. The nicotinic cholinergic receptor : different compositions evidenced by statistical analysis.
    Biochem Biophys Res Commun. 1975 Jan 20;62(2):407-14 PMID: 1111530
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1990457
Published
1981-11-01
Pages
457-60
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1163391
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1990457 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2