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PMID: 7329454 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The effects of barium on the electrical properties of the basolateral membrane in proximal tubule.

Naunyn-Schmiedeberg's archives of pharmacology ·Vol. 318 ·No. 2 ·1981-12-00 ·Pages 135-41

Planelles G, Teulon J, Anagnostopoulos T

Abstract

We studied the effects of millimolar Ba2+ concentrations on the properties of the basolateral membrane of the proximal tubule in Necturus kidney. Ba2+ was added in the peritubular perfusate by means of capillary microperfusion experiments. Basolateral membrane p.d. and conductance were continuously monitored in single tubules (their lumen was filled with oil) during reversible application of Ba2+ in peritubular fluid. The effect was membrane depolarization by 14mV and a decrease of membrane conductance to 63% of control values. This association strongly suggests that the main effect of Ba2+ is a decrease of the partial conductance to K+ at the basolateral membrane. However, in other experiments, performed also in oil-filled tubules, Ba2+ was shown to decrease intracellular K+ activity, recorded continuously by means of double-barreled (selective vs non selective) K+ microelectrodes. This and other, indirect evidence support the hypothesis that Ba2+ elicits also a small increase of the partial conductance to sodium at the basolateral membrane.

MeSH Terms
Animals Barium/pharmacology Capillaries/physiology Cell Membrane/physiology Electrophysiology Kidney Tubules, Proximal/drug effects Membrane Potentials/drug effects Membranes/drug effects Necturus Neural Conduction/drug effects Potassium/physiology
Chemicals
Barium Potassium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Planelles G
Teulon J
Anagnostopoulos T
References (26)
26 references, click to expand
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Article Info
Journal
Naunyn-Schmiedeberg's archives of pharmacology
Abbr.
Naunyn Schmiedebergs Arch Pharmacol
ISSN
0028-1298
DOI
10.1007/BF00508838
Published
1981-12-00
Pages
135-41
Language
English
Region
Germany
NLM ID
0326264
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00508838 View journal details
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