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PMID: 7306039 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Differential expression of alpha-lactalbumin and casein genes during the onset of lactation in the guinea-pig mammary gland.

The Biochemical journal ·Vol. 194 ·No. 3 ·1981-03-15 ·Pages 999-1006

Burditt LJ, Parker D, Craig RK, Getova T, Campbell PN

Abstract

1. The expression of alpha-lactalbumin and casein genes was examined in guinea-pig mammary tissue taken from animals both pre- and post-partum. 2. Analysis of total RNA by RNA excess hybridization with sequence-specific complementary DNA probes demonstrated that alpha-lactalbumin mRNA was present late in pregnancy, and that maximum concentrations were present at parturition. Casein gene transcripts were absent late in pregnancy (62 days), but by parturition were present at concentrations identical to those found at all time points examined throughout lactation. 3. Studies using mammary explants in organ culture showed that tissue from pregnant animals, or animals at parturition, synthesized and secreted only alpha-lactalbumin. After parturition, at the onset of casein synthesis, differential rates of secretion of alpha-lactalbumin and the caseins were observed. 4. The results are discussed in terms of the multiple intracellular mechanisms involved in the regulation of milk protein gene expression in the guinea-pig mammary gland.

MeSH Terms
Animals Caseins/biosynthesis,genetics Electrophoresis, Polyacrylamide Gel Female Gene Expression Regulation Guinea Pigs Lactalbumin/biosynthesis,genetics Lactation Mammary Glands, Animal/metabolism Organ Culture Techniques Pregnancy RNA, Messenger/genetics,metabolism Transcription, Genetic
Chemicals
Caseins RNA, Messenger Lactalbumin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Burditt L J
Parker D
Craig R K
Getova T
Campbell P N
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33 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1940999
Published
1981-03-15
Pages
999-1006
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1162838
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1940999 PMC Full Text View journal details
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