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PMID: 7305952 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An easy method for the determination of initial rates.

The Biochemical journal ·Vol. 193 ·No. 3 ·1981-03-01 ·Pages 1009-12

Waley SG

Abstract

When the Michaelis-Menten equation is obeyed, the rate near the beginning of an enzyme-catalyzed reaction (or of an experiment on transport) can be found accurately from the slope of a chord joining two points on the progress curve. This slope gives the rate at an intermediate concentration. Exact values of this intermediate concentration are easily calculated from equations in the text, and a number of values have also been tabulated. Methods of using two chords to find the initial rate are given. A mid-point formula for numerical differentiation is advocated when the Michaelis-Menten equation does not hold.

MeSH Terms
Biological Transport Catalysis Enzymes/metabolism Kinetics Methods Statistics as Topic
Chemicals
Enzymes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Waley S G
References (13)
13 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1931009
Published
1981-03-01
Pages
1009-12
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1162697
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1931009 PMC Full Text View journal details
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