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PMID: 7295298 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

An allele (Pk-1b) from wild-caught mice that affects the activity and kinetics of erythrocyte and liver pyruvate kinase.

Biochemical genetics ·Vol. 19 ·No. 7-8 ·1981-08-00 ·Pages 771-81

Moore KJ, Bulfield G

Abstract

A true breeding strain was made from a wild-caught mouse with low erythrocyte pyruvate kinase (E.C. 2.7.1.40) activity. This variation showed additive inheritance and segregated as an allele at a single locus (Pk-1b). Mice homozygous for the reduced blood pyruvate kinase activity cosegregated for reduced liver activity. In both these tissues the variant enzyme had a lowered heat stability and reduced Km values for ADP. An increased stimulation by FDP was also detected in the liver pyruvate kinase. No difference in the isoelectric point of the variant enzyme in either erythrocyte or liver was observed when compared with the enzyme from C57BL mice (Pk-1a/Pk-1a). It is concluded that Pk-1 is the structural gene for the erythrocyte and the major liver pyruvate kinase. No other tissue pyruvate kinase showed altered characteristics.

MeSH Terms
Alleles Animals Erythrocytes/enzymology Genetic Variation Liver/enzymology Male Mice Mice, Inbred C57BL Organ Specificity Pyruvate Kinase/genetics,metabolism Species Specificity
Chemicals
Pyruvate Kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Moore K J
Bulfield G
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23 references, click to expand
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Article Info
Journal
Biochemical genetics
Abbr.
Biochem Genet
ISSN
0006-2928
DOI
10.1007/BF00484008
Published
1981-08-00
Pages
771-81
Language
English
Region
United States
NLM ID
0126611
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00484008 View journal details
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