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PMID: 7287816 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Five-hour half-life of mouse liver gap-junction protein.

The Journal of cell biology ·Vol. 90 ·No. 2 ·1981-08-00 ·Pages 521-6

Fallon RF, Goodenough DA

Abstract

The half-life of a gap-junction polypeptide band migrating at 21,000 Mr on SDS polyacrylamide gels isolated from mouse liver is measured to be 5 h. Two low-molecular wight bands, probably related to the 21,000 Mr material by proteolysis, have measured half-lives of 4.6 and 5.2 h. Gap junctions are labeled in vivo using the 14C-bicarbonate labeling procedure, followed by quantitative fluorography.

MeSH Terms
Animals Half-Life Intercellular Junctions/metabolism Liver/metabolism,ultrastructure Mice Molecular Weight Proteins/metabolism
Chemicals
Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fallon R F
Goodenough D A
References (37)
37 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.90.2.521
Published
1981-08-00
Pages
521-6
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2111874
Subset
IM
Grants
NIGMS NIH HHS · GM 18974 · United States
External Links
PubMed source DOI: 10.1083/jcb.90.2.521 PMC Full Text View journal details
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