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PMID: 7286979 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The chromosomal distribution of repetitive DNA sequences within the human beta globin gene cluster.

Human genetics ·Vol. 57 ·No. 4 ·1981-00-00 ·Pages 388-93

Malcolm S, Barton P, Ferguson-Smith MA

Abstract

The chromosomal distribution and degree of repetitiveness of the sequences within the human beta globin gene cluster has been studied by in situ hybridisation. A genomic recombinant, H beta G1, which contains 15.9 thousand base pairs (kb) of DNA inserted into lambda charon 4A, was used as a template for the [3H] complementary RNA used for hybridisation. The inserted DNA contains sequences 4.7 kb to the 5' side of the delta gene and continues through the delta and beta genes to a site 2.8 kb to the 3' side of the beta gene. Two highly repetitive sequences, which are distributed evenly over all the chromosomes, have been identified within this DNA. Another somewhat less repetitive sequence has been identified between the beta and delta genes.

MeSH Terms
Base Sequence Chromosome Mapping DNA/genetics DNA Replication Genes Globins/genetics Humans In Vitro Techniques Nucleic Acid Hybridization RNA, Viral/genetics
Chemicals
RNA, Viral Globins DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Malcolm S
Barton P
Ferguson-Smith M A
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26 references, click to expand
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Article Info
Journal
Human genetics
Abbr.
Hum Genet
ISSN
0340-6717
DOI
10.1007/BF00281691
Published
1981-00-00
Pages
388-93
Language
English
Region
Germany
NLM ID
7613873
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00281691 View journal details
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