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题目:: Cell cycle parameters of 3T3 cells cultured as aggregates.
作者:: Gershman(H),Crissman(H A),Carrino(D A)
状态:: 发布时间1981-11-19 , 更新时间 2009-11-11
期刊:: In Vitro
摘要:: BALB/c3T3 cells cultured as aggregates were examined by two independent techniques to determine whether or not cells accumulated at a specific point in the cell cycle, and if so to determine the point at which they accumulate. Replating cells onto dishes followed by pulse labeling with [3H]thymidine and autoradiography indicated that aggregate-cultured cells were in the same phase of the cell cycle as cells cultured as confluent monolayers. Flow microfluorometry confirmed that 75% of the aggregate-maintained cells were arrested in G(0) or G(1), with 25% distributed throughout the rest of the cell cycle. Labeling and mitotic indices of cells in aggregates were also consistent with about 20 to 25% of the cells being in S + G(2) = M phases of the cell cycle at any time.
语言:: eng
DOI:

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