Human monoclonal antibodies to lung-cancer antigens.

British journal of cancer ·Vol. 43 ·No. 5 ·1981-05-00 ·Pages 696-700

Sikora K, Wright R

Abstract

Lymphocytes obtained from hilar and bronchial lymph nodes from 23 patients undergoing radical surgery for carcinoma of the bronchus were fused with established rat or mouse myeloma lines. 62% of the resultant hybrids were found to be secreting human Ig detected by a sensitive staphylococcal Protein A-coupled SRBC assay. Immunoglobulins synthesized by such hybrids were internally labelled with 3H-lysine and their antibody activity against a variety of membrane preparations determined. Nine monoclonal antibodies were found which bound to molecules on lung-cancer membranes and not on normal lung membranes from the same patient.

MeSH Terms

Aged Animals Antibodies, Neoplasm/biosynthesis Antibody Specificity Antigen-Antibody Reactions Antigens, Neoplasm/immunology Antigens, Surface/immunology Female Humans Hybrid Cells Immunoglobulins/biosynthesis Lung Neoplasms/immunology Lymphocytes/immunology Male Mice Middle Aged Rats

Chemicals

Antibodies, Neoplasm Antigens, Neoplasm Antigens, Surface Immunoglobulins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Sikora K

Wright R

References (14)

14 references, click to expand

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Article Info

Journal

British journal of cancer

Abbr.

Br J Cancer

ISSN

0007-0920

DOI

10.1038/bjc.1981.101

Published

1981-05-00

Pages

696-700

Language

English

Region

England

NLM ID

0370635

PMCID

PMC2010695

Subset

External Links

PubMed source DOI: 10.1038/bjc.1981.101 PMC Full Text View journal details

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Human monoclonal antibodies to lung-cancer antigens.

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