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PMID: 724514 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Histone-DNA interactions within chromatin. Isolation of histones from DNA-histone adducts induced in nuclei by UV light.

Nucleic acids research ·Vol. 5 ·No. 11 ·1978-11-00 ·Pages 4263-72

Kunkel GR, Martinson HG

Abstract

We have developed a method by which to isolate histones that have been crosslinked to DNA following irradiation of calf thymus nuclei by UV light. The procedure involves separation of protein-DNA adducts from uncrosslinked protein by Sepharose 4B chromatography under dissociating conditions. Histones which are crosslinked to DNA are released by chemical hydrolysis of the DNA and identified by SDS gel electrophoresis. The results indicate that, of the histones, H1 and H3 become crosslinked to the DNA most readily under our irradiation conditions.

MeSH Terms
Animals Cattle Cell Nucleus/radiation effects Chromatin/radiation effects DNA/radiation effects Histones/radiation effects Molecular Weight Protein Binding Thymus Gland Ultraviolet Rays
Chemicals
Chromatin Histones DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kunkel G R
Martinson H G
References (16)
16 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.11.4263
Published
1978-11-00
Pages
4263-72
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342747
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/5.11.4263 PMC Full Text View journal details
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