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PMID: 7241576 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ion transport in 'tight' epithelial monolayers of MDCK cells.

The Journal of membrane biology ·Vol. 59 ·No. 2 ·1981-04-15 ·Pages 105-14

Simmons NL

Abstract

Cultured monolayers of MDCK cells grown upon filter supports display many features of in vivo epithelia. Previously reported values of transmonolayer resistance of 100 omega cm-2 (Misfeldt, Hamamoto & Pitelka, 1976; Cereijido, Robbins, Dolan, Rotunno & Sabatini, 1978) indicate a leaky epithelium. This paper describes the properties of a strain of MDCK cells which displays entirely different electrophysiological properties. The results show that (i) the mean transmonolayer resistance is 4.16 k omega cm-2, (ii) transmonolayer ion transport is of small magnitude since the mean spontaneous open circuit PD is only 2.17 mV basal surface positive and isotopic Na and Cl flux measurements fail to demonstrate a significant net flux, (iii) the action of ouabain, amiloride and ion substitutions are consistent with transmonolayer net Na movement being largely responsible for the spontaneous PD, and (iv) asymmetry in the localization of the Na-K ATPase is evident on the basis of 3H-ouabain binding to cell monolayer.

MeSH Terms
Animals Biological Transport Cell Line Dogs Electric Conductivity Epithelium/physiology,ultrastructure Intercellular Junctions/physiology Ions/metabolism Kidney Membrane Potentials Ouabain/pharmacology
Chemicals
Ions Ouabain
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Simmons N L
References (33)
33 references, click to expand
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01875708
Published
1981-04-15
Pages
105-14
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01875708 View journal details
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