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题目:: Separation of AKR mouse thymus lymphoma from normal thymic cells by centrifugal elutriation.
作者:: Meistrich(M L),Nell(L J),Richie(E S)
状态:: 发布时间1981-07-23 , 更新时间 2007-11-14
期刊:: J Immunol Methods
摘要:: The rapid separation of large numbers of viable thymus cells from AKR mice bearing transplanted or spontaneous thymic lymphomas was achieved by centrifugal elutriation. Separation of more than 3 x 10(8) cells from either a transplanted lymphoma, designated 720, or from a spontaneous thymic lymphoma, required only 15 min. Unfractionated thymus cells obtained from mice bearing the transplanted lymphoma consisted of 80% lymphoma cells (by immunofluorescence for the viral protein, gp70) and 20% normal cells. Fractions of slowly sedimenting cells consisted almost exclusively of normal cells (95%) with modal volumes of 95 micrometers cubed. Fractions of rapidly sedimenting cells consisted of 95% tumor cells with volumes of 150-400 micrometers cubed. The slowly sedimenting cells were almost exclusively (98%) in the GO- or G1-phase. Fractions of rapidly sedimenting cells contained up to 55% S-phase and up to 30% G2-phase cells. Intermediate fractions contained mixtures of normal cells and small GO- or G1-phase tumor cells. Thymidine uptake by the separated cells was determined. The fractions containing normal cells showed little thymidine uptake after 4 and 48 h in culture, while the fractions of tumor cells showed high levels of incorporation. In contrast to the high levels of thymidine uptake by the tumor cell fractions after 48 h in culture, there was little uptake by the unseparated cell suspension, suggesting a possible interaction between normal and tumor cells during the culture period.
语言:: eng
DOI:

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