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PMID: 7220346 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

IN vitro transcription of bacteriophage T4 tRNA gene cluster from two different promoters.

Nucleic acids research ·Vol. 9 ·No. 3 ·1981-02-11 ·Pages 519-27

Goldfarb A

Abstract

Analysis of primary transcripts made by Escherichia coli RNA polymerase on T4 DNA containing an intact or partially deleted tRNA gene cluster demonstrates that the T4 tRNA genes are transcribed from two promoters differing in their strength. The stronger (P1) and the weaker (P2) promoters are located at distances of 1 kb and 1.5 kb from the tRNA genes, respectively. Selective initiation of individual transcripts with dinucleotides shows that P1 and P2 promoters contain the sequences TAT and CAC respectively. The two-promoter organisation of the tRNA cluster may reflect two superimposed mechanisms of gene expression in T4-infected bacteria.

MeSH Terms
Chromosome Mapping DNA-Directed RNA Polymerases/metabolism Genes, Viral Operon RNA, Transfer/biosynthesis RNA, Viral/biosynthesis T-Phages/genetics Transcription, Genetic
Chemicals
RNA, Viral RNA, Transfer DNA-Directed RNA Polymerases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Goldfarb A
References (13)
13 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.3.519
Published
1981-02-11
Pages
519-27
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327219
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.3.519 PMC Full Text View journal details
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