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PMID: 72162 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

In vitro transcription of the avian retrovirus genome by the alpha form of the viral RNA-directed DNA polymerase.

Journal of virology ·Vol. 24 ·No. 2 ·1977-11-00 ·Pages 704-8

Collett MS, Grandgenett DP, Faras AJ

Abstract

The nature of transcription of the avian retrovirus RNA genome by the alpha form of the viral RNA-directed DNA polymerase has been investigated. Transcription was most efficient when Mn2+ was provided as the divalent metal ion. The patterns of DNA transcription using 70S RNA, 35S RNA-tRNAtrp, or 35S RNA-oligo(dT)12-18 template-primer complexes by the alpha DNA polymerase were essentially identical to those obtained using the alphabeta form. The alpha DNA polymerase appears to be deficient in the synthesis of true duplex DNA but is able to synthesize hairpin-structured DNA initiated at the 5' terminus of the viral genome on the tRNAtrp primer molecule.

MeSH Terms
Avian Leukosis Virus/genetics Avian Myeloblastosis Virus/genetics DNA, Viral/biosynthesis,genetics Genes, Viral Manganese/pharmacology Nucleic Acid Conformation RNA, Transfer/genetics RNA, Viral/genetics RNA-Directed DNA Polymerase/metabolism Structure-Activity Relationship Transcription, Genetic
Chemicals
DNA, Viral RNA, Viral Manganese RNA, Transfer RNA-Directed DNA Polymerase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Collett M S
Grandgenett D P
Faras A J
References (35)
35 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.24.2.704-708.1977
Published
1977-11-00
Pages
704-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515984
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.24.2.704-708.1977 PMC Full Text View journal details
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