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PMID: 7213345 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The electrophoresis of transferrins in urea/polyacrylamide gels.

The Biochemical journal ·Vol. 189 ·No. 3 ·1980-09-01 ·Pages 541-6

Evans RW, Williams J

Abstract

The denaturation of transferrin by urea has been studied by (a) electrophoresis in polyacrylamide gels incorporating a urea gradient, (b) measurements of the loss of iron-binding capacity and (c) u.v. difference spectrometry. In human serum transferrin and hen ovotransferrin the N-terminal and C-terminal domains of the iron-free protein were found to denature at different urea concentrations.

MeSH Terms
Animals Chickens Conalbumin Electrophoresis, Polyacrylamide Gel Humans Iron Protein Binding Protein Denaturation Spectrophotometry, Ultraviolet Transferrin Urea
Chemicals
Transferrin Conalbumin Urea Iron
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Evans R W
Williams J
References (14)
14 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1890541
Published
1980-09-01
Pages
541-6
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1162034
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1890541 PMC Full Text View journal details
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